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dc.contributor.authorAslam, Nazneen
dc.contributor.authorAbusharieh, Elham
dc.contributor.authorAbuarqoub, Duaa
dc.contributor.authorAli, Dema
dc.contributor.authorAlhattab, Dana Majed
dc.contributor.authorWehaibi, Suha
dc.contributor.authorAl-Kurdi, Ban
dc.contributor.authorJamali, Fatima
dc.contributor.authorAlshaer, Walhan
dc.contributor.authorJafar, Hanan
dc.contributor.authorAwidi, Abdalla S.
dc.date.accessioned2021-02-04T07:56:00Z
dc.date.available2021-02-04T07:56:00Z
dc.date.issued2020-11-26
dc.date.submitted2020-08-04
dc.identifier.citationAslam, N., Abusharieh, E., Abuarqoub, D., Ali, D., Al-Hattab, D., Wehaibi, S., … Awidi, A. S. (2021). Anti-oncogenic activities exhibited by paracrine factors of MSCs can be mediated by modulation of KITLG and DKK1 genes in glioma SCs in vitro. Molecular Therapy - Oncolytics, 20, 147–165. doi:10.1016/j.omto.2020.11.005
dc.identifier.issn2372-7705
dc.identifier.doi10.1016/j.omto.2020.11.005
dc.identifier.urihttp://hdl.handle.net/10754/667215
dc.description.abstractCancer stem cells (CSCs) use their stemness properties to perpetuate their lineage and survive chemotherapy. Currently cell-based and cell-free therapies are under investigation to develop novel anti-cancer treatment modalities. We designed this study to investigate how cell extracts of mesenchymal stem cells affect the growth of glioma stem cells in vitro. Gliospheres were generated from the U87MG cell line and treated with conditioned media of Wharton's jelly and bone marrow mesenchymal stem cells. The effects were investigated at the functional and molecular levels. Our results showed that conditioned media from both types of mesenchymal stem cells changed the morphology of spheres and inhibited the proliferation, invasion, and self-renewal ability of glioma stem cells. At the molecular level, metabolism interruption at oxidative phosphorylation, cell cycle arrest, cell differentiation, and upregulation of the immune response were observed. Furthermore, this effect was mediated by the upregulation of the DKK1 gene inhibiting the Wnt pathway mediated by growth factor activity and downregulation of the KITLG gene activated by growth factor and cytokine activity, inhibiting multiple pathways. We conclude that different types of mesenchymal stem cells possess antitumor properties and their paracrine factors, in combination with anti-immune modalities, can provide practical therapeutic targets for glioblastoma treatment.
dc.description.sponsorshipWe are thankful to all the participants and management team at the Cell Therapy Center for their continuous cooperation and funding support for this study. We extend our gratitude to Dr Noor Gammoh from the University of Edinburgh, whose valuable comments make the manuscript clear and presentable. The graphical abstract was created with BioRender (https://biorender.com/). Conceptualization and Design, N.A.; Methodology, N.A. and E.A.; Formal Analysis, N.A. and D.A.; Investigation, N.A. E.A. D. Ali, S.W. and B.A.; Resources, D.A.-H. ; Data Curation, W.A.; Writing – Original Draft, N.A.; Writing – Review & Editing, F.J.; Project Administration, H.J. and A.A.; Funding Acquisition, A.A. The authors declare no competing interests.
dc.publisherElsevier BV
dc.relation.urlhttps://linkinghub.elsevier.com/retrieve/pii/S2372770520301704
dc.rightsThis is an open access article under the CC BY license.
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleAnti-oncogenic activities exhibited by paracrine factors of MSCs can be mediated by modulation of KITLG and DKK1 genes in glioma SCs in vitro
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalMolecular Therapy - Oncolytics
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionCell Therapy Center, The University of Jordan, Amman 11942, Jordan
dc.contributor.institutionFaculty of Pharmacy, The University of Jordan, Amman 11942, Jordan
dc.contributor.institutionDepartment of Pharmacology and Biomedical Sciences, Faculty of Pharmacy and Medical Sciences, University of Petra, Amman 11196, Jordan
dc.contributor.institutionDepartment of Anatomy and Histology, School of Medicine, The University of Jordan, Amman 11942, Jordan
dc.contributor.institutionDepartment of Medicine, School of Medicine, The University of Jordan, Amman 11942, Jordan
dc.contributor.institutionDepartment of Hematology and Oncology, Jordan University Hospital, The University of Jordan, Amman 11942, Jordan
dc.identifier.volume20
dc.identifier.pages147-165
kaust.personAlhattab, Dana Majed
dc.date.accepted2020-11-19
dc.identifier.eid2-s2.0-85099961080
refterms.dateFOA2021-02-04T07:57:20Z


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