Hadamard-transform spectral acquisition with an acousto-optic tunable filter in a broadband stimulated Raman scattering microscope
Biological and Environmental Sciences and Engineering (BESE) Division
Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia
KAUST Grant NumberOSR-2016-CRG5-3017
Permanent link to this recordhttp://hdl.handle.net/10754/667187
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AbstractWe present a novel configuration for high spectral resolution multiplexing acquisition based on the Hadamard transform in stimulated Raman scattering (SRS) microscopy. The broadband tunable output of a dual-beam femtosecond laser is filtered by a fast, narrowband, and multi-channel acousto-optic tunable filter (AOTF). By turning on and off different subsets of its 8 independent channels, the AOTF generates the spectral masks given by the Hadamard matrix. We demonstrate a seamless and automated operation in the Raman fingerprint and CH-stretch regions. In the presence of additive noise, the spectral measurements using the multiplexed method show the same signal-to-noise ratio of conventional single-wavenumber acquisitions performed with 4 times longer integration time.
CitationGenchi, L., Bucci, A., Laptenok, S. P., Giammona, A., & Liberale, C. (2021). Hadamard-transform spectral acquisition with an acousto-optic tunable filter in a broadband stimulated Raman scattering microscope. Optics Express, 29(2), 2378. doi:10.1364/oe.415752
SponsorsKing Abdullah University of Science and Technology (OSR-2016-CRG5-3017).
PublisherThe Optical Society