Hadamard-transform spectral acquisition with an acousto-optic tunable filter in a broadband stimulated Raman scattering microscope
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Label-Free Optical Microscopy for Biology Lab
KAUST Grant NumberOSR-2016-CRG5-3017
Online Publication Date2021-01-13
Print Publication Date2021-01-18
Permanent link to this recordhttp://hdl.handle.net/10754/667187
MetadataShow full item record
AbstractWe present a novel configuration for high spectral resolution multiplexing acquisition based on the Hadamard transform in stimulated Raman scattering (SRS) microscopy. The broadband tunable output of a dual-beam femtosecond laser is filtered by a fast, narrowband, and multi-channel acousto-optic tunable filter (AOTF). By turning on and off different subsets of its 8 independent channels, the AOTF generates the spectral masks given by the Hadamard matrix. We demonstrate a seamless and automated operation in the Raman fingerprint and CH-stretch regions. In the presence of additive noise, the spectral measurements using the multiplexed method show the same signal-to-noise ratio of conventional single-wavenumber acquisitions performed with 4 times longer integration time.
CitationGenchi, L., Bucci, A., Laptenok, S. P., Giammona, A., & Liberale, C. (2021). Hadamard-transform spectral acquisition with an acousto-optic tunable filter in a broadband stimulated Raman scattering microscope. Optics Express, 29(2), 2378. doi:10.1364/oe.415752
SponsorsKing Abdullah University of Science and Technology (OSR-2016-CRG5-3017).
PublisherThe Optical Society