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    Hadamard-transform spectral acquisition with an acousto-optic tunable filter in a broadband stimulated Raman scattering microscope

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    Type
    Article
    Authors
    Genchi, Luca cc
    Bucci, Andrea
    Laptenok, Siarhei cc
    Giammona, Alessandro
    Liberale, Carlo cc
    KAUST Department
    Bioscience
    Biological and Environmental Sciences and Engineering (BESE) Division
    Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia
    Bioscience Program
    KAUST Grant Number
    OSR-2016-CRG5-3017
    Date
    2021-01-13
    Submitted Date
    2020-11-23
    Permanent link to this record
    http://hdl.handle.net/10754/667187
    
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    Abstract
    We present a novel configuration for high spectral resolution multiplexing acquisition based on the Hadamard transform in stimulated Raman scattering (SRS) microscopy. The broadband tunable output of a dual-beam femtosecond laser is filtered by a fast, narrowband, and multi-channel acousto-optic tunable filter (AOTF). By turning on and off different subsets of its 8 independent channels, the AOTF generates the spectral masks given by the Hadamard matrix. We demonstrate a seamless and automated operation in the Raman fingerprint and CH-stretch regions. In the presence of additive noise, the spectral measurements using the multiplexed method show the same signal-to-noise ratio of conventional single-wavenumber acquisitions performed with 4 times longer integration time.
    Citation
    Genchi, L., Bucci, A., Laptenok, S. P., Giammona, A., & Liberale, C. (2021). Hadamard-transform spectral acquisition with an acousto-optic tunable filter in a broadband stimulated Raman scattering microscope. Optics Express, 29(2), 2378. doi:10.1364/oe.415752
    Sponsors
    King Abdullah University of Science and Technology (OSR-2016-CRG5-3017).
    Publisher
    The Optical Society
    Journal
    Optics Express
    DOI
    10.1364/OE.415752
    Additional Links
    https://www.osapublishing.org/abstract.cfm?URI=oe-29-2-2378
    ae974a485f413a2113503eed53cd6c53
    10.1364/OE.415752
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program

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