Hadamard-transform spectral acquisition with an acousto-optic tunable filter in a broadband stimulated Raman scattering microscope
Type
ArticleKAUST Department
BioscienceBiological and Environmental Sciences and Engineering (BESE) Division
Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia
Bioscience Program
KAUST Grant Number
OSR-2016-CRG5-3017Date
2021-01-13Submitted Date
2020-11-23Permanent link to this record
http://hdl.handle.net/10754/667187
Metadata
Show full item recordAbstract
We present a novel configuration for high spectral resolution multiplexing acquisition based on the Hadamard transform in stimulated Raman scattering (SRS) microscopy. The broadband tunable output of a dual-beam femtosecond laser is filtered by a fast, narrowband, and multi-channel acousto-optic tunable filter (AOTF). By turning on and off different subsets of its 8 independent channels, the AOTF generates the spectral masks given by the Hadamard matrix. We demonstrate a seamless and automated operation in the Raman fingerprint and CH-stretch regions. In the presence of additive noise, the spectral measurements using the multiplexed method show the same signal-to-noise ratio of conventional single-wavenumber acquisitions performed with 4 times longer integration time.Citation
Genchi, L., Bucci, A., Laptenok, S. P., Giammona, A., & Liberale, C. (2021). Hadamard-transform spectral acquisition with an acousto-optic tunable filter in a broadband stimulated Raman scattering microscope. Optics Express, 29(2), 2378. doi:10.1364/oe.415752Sponsors
King Abdullah University of Science and Technology (OSR-2016-CRG5-3017).Publisher
The Optical SocietyJournal
Optics ExpressAdditional Links
https://www.osapublishing.org/abstract.cfm?URI=oe-29-2-2378ae974a485f413a2113503eed53cd6c53
10.1364/OE.415752