Lycopene β-cyclase expression influences plant physiology, development and metabolism in tobacco plants
Perez da Sousa, Leonardo
Aurel Schottler, Mark
Moreno, Juan C
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Desert Agriculture Initiative
King Abdullah University of Science and Technology, Division of Biological and Environmental Sciences and Engineering, Center for Desert Agriculture, the BioActives Lab, Thuwal, Kingdom of Saudi Arabia
Permanent link to this recordhttp://hdl.handle.net/10754/667054
MetadataShow full item record
AbstractAbstract Carotenoids are important isoprenoids produced in the plastids of photosynthetic organisms that play key roles in photoprotection and antioxidative processes. β-carotene is generated from lycopene by the lycopene β-cyclase (LCYB). Previously, we demonstrated that the introduction of the Daucus carota (carrot) DcLCYB1 gene into tobacco (cultivar Xanthi) resulted in increased levels of abscisic acid (ABA) and especially gibberellins (GAs), resulting in increased plant yield. In order to understand this phenomenon prior exporting this genetic strategy to crops, we generated tobacco (cultivar Petit Havana) mutants that exhibited a wide range of LCYB expression. Transplastomic plants expressing DcLCYB1 at high levels showed a wild-type-like growth, even though their pigment content was increased, and their leaf GA content was reduced. RNAi NtLCYB lines showed different reductions in NtLCYB transcript abundance, correlating with reduced pigment content and plant variegation. Photosynthesis (leaf absorptance, Fv/Fm, and ETRII) and plant growth were impaired. Remarkably, drastic changes in phytohormone content also occurred in the RNAi lines. However, external application of phytohormones was not sufficient to rescue their phenotypes, suggesting that altered photosynthetic efficiency might be another important factor explaining their reduced biomass. These results show that LCYB expression influences plant biomass by different mechanisms and suggests thresholds for LCYB expression levels that might be beneficial/detrimental for plant growth.
CitationKossler, S., Armarego-Marriott, T., Tarkowska, D., Tureckova, V., Agrawal, S., Mi, J., … Moreno, J. C. (2021). Lycopene β-cyclase expression influences plant physiology, development and metabolism in tobacco plants. Journal of Experimental Botany. doi:10.1093/jxb/erab029
SponsorsWe are extremely grateful to Dr. Aleksandra Skirycz for supporting an independent research project to be completed in her research group at the Max-Planck-Institut für Molekulare Pflanzenphysiologie (MPI-MP). Dr. Daniel Karcher (MPI-MP), kindly provided the constructs pDK325 and pDK326 used for generation of pJM36 and pJM37 transplastomic vectors. We are grateful to Prof. Dr. Lothar Willmitzer for his great support and advice, and the MPI-MP and the head of the Green Team Dr. Karin Köhl and its members (Britta Hausmann, Florian Hundert, Dirk Zerning and Laura Westpahl) for plant cultivation. The gibberellin measurements were supported by a European Regional Development Fund Project “Centre for Experimental Plant Biology” (No. CZ.02.1.01/0.0/0.0/16_019/0000738) and the Czech Science Foundation (18-10349S).
PublisherOxford University Press (OUP)
JournalJournal of Experimental Botany
Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
- Increased Nicotiana tabacum fitness through positive regulation of carotenoid, gibberellin and chlorophyll pathways promoted by Daucus carota lycopene β-cyclase (Dclcyb1) expression.
- Authors: Moreno JC, Cerda A, Simpson K, Lopez-Diaz I, Carrera E, Handford M, Stange C
- Issue date: 2016 Apr
- Expression of a carotenogenic gene allows faster biomass production by redesigning plant architecture and improving photosynthetic efficiency in tobacco.
- Authors: Moreno JC, Mi J, Agrawal S, Kössler S, Turečková V, Tarkowská D, Thiele W, Al-Babili S, Bock R, Schöttler MA
- Issue date: 2020 Sep
- Genetic modification of tomato with the tobacco lycopene β-cyclase gene produces high β-carotene and lycopene fruit.
- Authors: Ralley L, Schuch W, Fraser PD, Bramley PM
- Issue date: 2016 Sep 1
- Levels of lycopene β-cyclase 1 modulate carotenoid gene expression and accumulation in Daucus carota.
- Authors: Moreno JC, Pizarro L, Fuentes P, Handford M, Cifuentes V, Stange C
- Issue date: 2013
- Building the Synthetic Biology Toolbox with Enzyme Variants to Expand Opportunities for Biofortification of Provitamin A and Other Health-Promoting Carotenoids.
- Authors: Zhu K, Zheng X, Ye J, Jiang Q, Chen H, Mei X, Wurtzel ET, Deng X
- Issue date: 2020 Oct 28