Ultrashort Peptide Bioinks Support Automated Printing of Large-Scale Constructs Assuring Long-Term Survival of Printed Tissue Constructs
Type
ArticleAuthors
Susapto, Hepi Hari
Alhattab, Dana Majed

Abdelrahman, Sherin

Khan, Zainab
Alshehri, Salwa
Kahin, Kowther
Ge, Rui
Moretti, Manola

Emwas, Abdul-Hamid M.
Hauser, Charlotte

KAUST Department
Biological and Environmental Science and Engineering (BESE) DivisionBioscience Program
Computational Bioscience Research Center (CBRC)
Laboratory for Nanomedicine
NMR
Physical Science and Engineering (PSE) Division
Date
2021-01-25Online Publication Date
2021-01-25Print Publication Date
2021-04-14Embargo End Date
2022-01-25Submitted Date
2020-11-07Permanent link to this record
http://hdl.handle.net/10754/667000
Metadata
Show full item recordAbstract
We report about rationally designed ultrashort peptide bioinks, overcoming severe limitations in current bioprinting procedures. Bioprinting is increasingly relevant in tissue engineering, regenerative and personalized medicine due to its ability to fabricate complex tissue scaffolds through an automated deposition process. Printing stable large-scale constructs with high shape fidelity and enabling long-term cell survival are major challenges that most existing bioinks are unable to solve. Additionally, they require chemical or UV-cross-linking for the structure-solidifying process which compromises the encapsulated cells, resulting in restricted structure complexity and low cell viability. Using ultrashort peptide bioinks as ideal bodylike but synthetic material, we demonstrate an instant solidifying cell-embedding printing process via a sophisticated extrusion procedure under true physiological conditions and at cost-effective low bioink concentrations. Our printed large-scale cell constructs and the chondrogenic differentiation of printed mesenchymal stem cells point to the strong potential of the peptide bioinks for automated complex tissue fabrication.Citation
Susapto, H. H., Alhattab, D., Abdelrahman, S., Khan, Z., Alshehri, S., Kahin, K., … Hauser, C. A. E. (2021). Ultrashort Peptide Bioinks Support Automated Printing of Large-Scale Constructs Assuring Long-Term Survival of Printed Tissue Constructs. Nano Letters. doi:10.1021/acs.nanolett.0c04426Sponsors
This work was financially supported by King Abdullah University of Science and Technology. The authors acknowledge KAUST’s Seed Fund Grant and KAUST’s Innovation Fund awarded by KAUST’s Innovation and Economic Development.The authors acknowledge Jordy Homing Lam for advising on the MD simulations and Ali H. Aldoukhi for 3D bioprinting support. We acknowledge the strong support from Rachid Sougrat, Ali R. Behzad, and Lingyun Zhao, KAUST Core Laboratories. We also acknowledge Walaa Alsanie, Raed Felimban, and Adeel Chaudhary for providing the mouse embryonic neuronal cells.
Publisher
American Chemical Society (ACS)Journal
Nano Lettersae974a485f413a2113503eed53cd6c53
10.1021/acs.nanolett.0c04426