Large-scale genome sequencing reveals the driving forces of viruses in microalgal evolution.
AuthorsNelson, David R
Hazzouri, Khaled M
Lauersen, Kyle J
Preston, Michael J
Lomas, Michael W
Amiri, Khaled M A
KAUST DepartmentBiological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Kingdom of Saudi Arabia.
Embargo End Date2022-01-12
Permanent link to this recordhttp://hdl.handle.net/10754/666949
MetadataShow full item record
AbstractBeing integral primary producers in diverse ecosystems, microalgal genomes could be mined for ecological insights, but representative genome sequences are lacking for many phyla. We cultured and sequenced 107 microalgae species from 11 different phyla indigenous to varied geographies and climates. This collection was used to resolve genomic differences between saltwater and freshwater microalgae. Freshwater species showed domain-centric ontology enrichment for nuclear and nuclear membrane functions, while saltwater species were enriched in organellar and cellular membrane functions. Further, marine species contained significantly more viral families in their genomes (p = 8e-4). Sequences from Chlorovirus, Coccolithovirus, Pandoravirus, Marseillevirus, Tupanvirus, and other viruses were found integrated into the genomes of algal from marine environments. These viral-origin sequences were found to be expressed and code for a wide variety of functions. Together, this study comprehensively defines the expanse of protein-coding and viral elements in microalgal genomes and posits a unified adaptive strategy for algal halotolerance.
CitationNelson, D. R., Hazzouri, K. M., Lauersen, K. J., Jaiswal, A., Chaiboonchoe, A., Mystikou, A., … Salehi-Ashtiani, K. (2021). Large-scale genome sequencing reveals the driving forces of viruses in microalgal evolution. Cell Host & Microbe. doi:10.1016/j.chom.2020.12.005
SponsorsWe thank the NYUAD High-Performance Computing Center for providing computational resources. This work was supported by Tamkeen under the NYU Abu Dhabi Research Institute grant to the NYUAD Center for Genomics and Systems Biology (73 71210 CGSB9), and by NYUAD Faculty Research Funds (AD060).
JournalCell host & microbe
- dEMBF: A Comprehensive Database of Enzymes of Microalgal Biofuel Feedstock.
- Authors: Misra N, Panda PK, Parida BK, Mishra BK
- Issue date: 2016
- Towards defining the chloroviruses: a genomic journey through a genus of large DNA viruses.
- Authors: Jeanniard A, Dunigan DD, Gurnon JR, Agarkova IV, Kang M, Vitek J, Duncan G, McClung OW, Larsen M, Claverie JM, Van Etten JL, Blanc G
- Issue date: 2013 Mar 8
- Illumina MiSeq Analysis and Comparison of Freshwater Microalgal Communities on Ulleungdo and Dokdo Islands.
- Authors: Yun HS, Kim YS, Yoon HS
- Issue date: 2019 Dec
- Insights into the evolution of vitamin B12 auxotrophy from sequenced algal genomes.
- Authors: Helliwell KE, Wheeler GL, Leptos KC, Goldstein RE, Smith AG
- Issue date: 2011 Oct
- The mitochondrial and chloroplast genomes of the haptophyte Chrysochromulina tobin contain unique repeat structures and gene profiles.
- Authors: Hovde BT, Starkenburg SR, Hunsperger HM, Mercer LD, Deodato CR, Jha RK, Chertkov O, Monnat RJ Jr, Cattolico RA
- Issue date: 2014 Jul 17