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dc.contributor.authorShekhawat, Kirti
dc.contributor.authorSaad, Maged
dc.contributor.authorSheikh, Arsheed Hussain
dc.contributor.authorMariappan, Kiruthiga
dc.contributor.authorAl-Mahmoudi, Henda
dc.contributor.authorabdulhakim, fatimah
dc.contributor.authorEida, Abdul Aziz
dc.contributor.authorJalal, Rewaa S.
dc.contributor.authorMasmoudi, Khaled
dc.contributor.authorHirt, Heribert
dc.date.accessioned2021-01-19T13:01:34Z
dc.date.available2021-01-19T13:01:34Z
dc.date.issued2021-01-10
dc.identifier.citationShekhawat, K., Saad, M. M., Sheikh, A., Mariappan, K., Al-Mahmoudi, H., Abdulhakim, F., … Hirt, H. (2021). Root endophyte induced plant thermotolerance by constitutive chromatin modification at heat stress memory gene loci. EMBO Reports. doi:10.15252/embr.202051049
dc.identifier.issn1469-221X
dc.identifier.issn1469-3178
dc.identifier.pmid33426785
dc.identifier.doi10.15252/embr.202051049
dc.identifier.urihttp://hdl.handle.net/10754/666935
dc.description.abstractGlobal warming has become a critical challenge to food security, causing severe yield losses of major crops worldwide. Conventional and transgenic breeding strategies to enhance plant thermotolerance are laborious and expensive. Therefore, the use of beneficial microbes could be an alternative approach. Here, we report that the root endophyte Enterobacter sp. SA187 induces thermotolerance in wheat in the laboratory as well as in open-field agriculture. To unravel the molecular mechanisms, we used Arabidopsis thaliana as model plant. SA187 reprogramed the Arabidopsis transcriptome via HSFA2-dependent enhancement of H3K4me3 levels at heat stress memory gene loci. Unlike thermopriming, SA187-induced thermotolerance is mediated by ethylene signaling via the transcription factor EIN3. In contrast to the transient chromatin modification by thermopriming, SA187 induces constitutive H3K4me3 modification of heat stress memory genes, generating robust thermotolerance in plants. Importantly, microbial community composition of wheat plants in open-field agriculture is not influenced by SA187, indicating that beneficial microbes can be a powerful tool to enhance thermotolerance of crops in a sustainable manner.
dc.description.sponsorshipWe would like to thank Dr. Ismahane Elouafi (ICBA) and Dr. Mohammad Shahid (ICBA) for the fruitful discussion and support during the field experiments as well as Dr. Marcela Herrera Sarrias and Dr. Manuel Aranda Lastra (KAUST) for guidance in the microbiome analysis. We would like to thank Dr. YY Charng for providing Arabidopsis seed mutants of hsfa2 and hsfa1-q. We thank Alexander Polussa for his technical support. We thank all members of the Hirt Lab, especially Dr. Naganand Rayapuram for his scientific suggestions and Olga Artyukh, the CDA management and greenhouse facility team of KAUST for the technical assistance. This publication is based upon work supported by the King Abdullah University of Science and Technology (KAUST), base fund for HH no. BAS/1/1062-01-01.
dc.publisherEMBO
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/10.15252/embr.202051049
dc.rightsPublished under the terms of the CC BY NC ND 4.0 license.
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleRoot endophyte induced plant thermotolerance by constitutive chromatin modification at heat stress memory gene loci
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentDARWIN21, Biological and Environmental Sciences and Engineering Division King Abdullah University of Science and Technology (KAUST) Thuwal Saudi Arabia
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentHirt Lab
dc.contributor.departmentPlant Science
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.identifier.journalEMBO reports
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionInternational Center for Biosaline Agriculture Academic CityNear Zayed University Dubai United Arab Emirates
dc.contributor.institutionDepartment of Biology College of Science University of Jeddah Jeddah Saudi Arabia
dc.contributor.institutionCollege of Food & Agriculture United Arab Emirates University Al Ain United Arab Emirates
dc.contributor.institutionMax Perutz Laboratories University of Vienna Vienna Austria
kaust.personShekhawat, Kirti
kaust.personSaad, Maged M
kaust.personSheikh, Arsheed Hussain
kaust.personMariappan, Kiruthiga
kaust.personAbdulhakim, Fatimah
kaust.personEida, Abdul Aziz
kaust.personHirt, Heribert
kaust.grant.numberBAS/1/1062
dc.relation.issupplementedbybioproject:PRJNA601157
dc.relation.issupplementedbybioproject:PRJNA615340
dc.identifier.eid2-s2.0-85099080708
refterms.dateFOA2021-01-19T13:02:50Z
display.relations<b>Is Supplemented By:</b><br/> <ul><li><i>[Bioproject]</i> <br/> Title: Molecular mechanisms of Enterobacter sp. SA187 induced thermotolerance in Arabidopsis thaliana. Publication Date: 2021-03-16. bioproject: <a href="https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA601157" >PRJNA601157</a> Handle: <a href="http://hdl.handle.net/10754/668130" >10754/668130</a></a></li><li><i>[Bioproject]</i> <br/> Title: Microbial communities of wheat plants inoculated with endophytic bacterium Enterobacter sp. SA187. Publication Date: 2021-03-16. bioproject: <a href="https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA615340" >PRJNA615340</a> Handle: <a href="http://hdl.handle.net/10754/668131" >10754/668131</a></a></li></ul>
kaust.acknowledged.supportUnitHirt Lab


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