Root endophyte induced plant thermotolerance by constitutive chromatin modification at heat stress memory gene loci
Sheikh, Arsheed Hussain
Eida, Abdul Aziz
Jalal, Rewaa S.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
DARWIN21, Biological and Environmental Sciences and Engineering Division King Abdullah University of Science and Technology (KAUST) Thuwal Saudi Arabia
Desert Agriculture Initiative
Red Sea Research Center (RSRC)
KAUST Grant NumberBAS/1/1062
Permanent link to this recordhttp://hdl.handle.net/10754/666935
MetadataShow full item record
AbstractGlobal warming has become a critical challenge to food security, causing severe yield losses of major crops worldwide. Conventional and transgenic breeding strategies to enhance plant thermotolerance are laborious and expensive. Therefore, the use of beneficial microbes could be an alternative approach. Here, we report that the root endophyte Enterobacter sp. SA187 induces thermotolerance in wheat in the laboratory as well as in open-field agriculture. To unravel the molecular mechanisms, we used Arabidopsis thaliana as model plant. SA187 reprogramed the Arabidopsis transcriptome via HSFA2-dependent enhancement of H3K4me3 levels at heat stress memory gene loci. Unlike thermopriming, SA187-induced thermotolerance is mediated by ethylene signaling via the transcription factor EIN3. In contrast to the transient chromatin modification by thermopriming, SA187 induces constitutive H3K4me3 modification of heat stress memory genes, generating robust thermotolerance in plants. Importantly, microbial community composition of wheat plants in open-field agriculture is not influenced by SA187, indicating that beneficial microbes can be a powerful tool to enhance thermotolerance of crops in a sustainable manner.
CitationShekhawat, K., Saad, M. M., Sheikh, A., Mariappan, K., Al-Mahmoudi, H., Abdulhakim, F., … Hirt, H. (2021). Root endophyte induced plant thermotolerance by constitutive chromatin modification at heat stress memory gene loci. EMBO Reports. doi:10.15252/embr.202051049
SponsorsWe would like to thank Dr. Ismahane Elouafi (ICBA) and Dr. Mohammad Shahid (ICBA) for the fruitful discussion and support during the field experiments as well as Dr. Marcela Herrera Sarrias and Dr. Manuel Aranda Lastra (KAUST) for guidance in the microbiome analysis. We would like to thank Dr. YY Charng for providing Arabidopsis seed mutants of hsfa2 and hsfa1-q. We thank Alexander Polussa for his technical support. We thank all members of the Hirt Lab, especially Dr. Naganand Rayapuram for his scientific suggestions and Olga Artyukh, the CDA management and greenhouse facility team of KAUST for the technical assistance. This publication is based upon work supported by the King Abdullah University of Science and Technology (KAUST), base fund for HH no. BAS/1/1062-01-01.
RelationsIs Supplemented By:
Title: Molecular mechanisms of Enterobacter sp. SA187 induced thermotolerance in Arabidopsis thaliana. Publication Date: 2021-03-16. bioproject: PRJNA601157 Handle: 10754/668130
Title: Microbial communities of wheat plants inoculated with endophytic bacterium Enterobacter sp. SA187. Publication Date: 2021-03-16. bioproject: PRJNA615340 Handle: 10754/668131
Except where otherwise noted, this item's license is described as Published under the terms of the CC BY NC ND 4.0 license.
- Ethylene induced plant stress tolerance by Enterobacter sp. SA187 is mediated by 2-keto-4-methylthiobutyric acid production.
- Authors: de Zélicourt A, Synek L, Saad MM, Alzubaidy H, Jalal R, Xie Y, Andrés-Barrao C, Rolli E, Guerard F, Mariappan KG, Daur I, Colcombet J, Benhamed M, Depaepe T, Van Der Straeten D, Hirt H
- Issue date: 2018 Mar
- Two interacting ethylene response factors regulate heat stress response.
- Authors: Huang J, Zhao X, Bürger M, Wang Y, Chory J
- Issue date: 2021 Apr 17
- HsfA2 Controls the Activity of Developmentally and Stress-Regulated Heat Stress Protection Mechanisms in Tomato Male Reproductive Tissues.
- Authors: Fragkostefanakis S, Mesihovic A, Simm S, Paupière MJ, Hu Y, Paul P, Mishra SK, Tschiersch B, Theres K, Bovy A, Schleiff E, Scharf KD
- Issue date: 2016 Apr
- Heat-response patterns of the heat shock transcription factor family in advanced development stages of wheat (Triticum aestivum L.) and thermotolerance-regulation by TaHsfA2-10.
- Authors: Guo XL, Yuan SN, Zhang HN, Zhang YY, Zhang YJ, Wang GY, Li YQ, Li GL
- Issue date: 2020 Aug 3
- Thermopriming reprograms metabolic homeostasis to confer heat tolerance.
- Authors: Serrano N, Ling Y, Bahieldin A, Mahfouz MM
- Issue date: 2019 Jan 17