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dc.contributor.authorRamaprasad, Abhinay
dc.contributor.authorSubudhi, Amit
dc.contributor.authorCulleton, Richard
dc.contributor.authorPain, Arnab
dc.date.accessioned2021-01-19T06:58:30Z
dc.date.available2021-01-19T06:58:30Z
dc.date.issued2018-10-01
dc.identifier.urihttp://hdl.handle.net/10754/666924
dc.description.abstractThe transcriptional regulation occurring in malaria parasites during the clinically important life stages within host erythrocytes can be studied in vivo with rodent malaria parasites propagated in mice. Time-series transcriptome profiling commonly involves the euthanasia of groups of mice at specific time points followed by the extraction of parasite RNA from whole blood samples. Current methodologies for parasite RNA extraction involve several steps and when multiple time points are profiled, these protocols are laborious, time consuming, and require the euthanisation of large cohorts of mice. We designed a simplified protocol for parasite RNA extraction from blood volumes as low as 20 microliters (microsamples), serially bled from mice via tail snips and directly lysed with TRIzol reagent. Gene expression data derived from microsampling using RNA-seq were closely matched to those derived from larger volumes of leucocyte depleted and saponin treated blood obtained from euthanized mice and also tightly correlated between biological replicates. Transcriptome profiling of microsamples taken at different time points during the intra-erythrocytic developmental cycle of the rodent malaria parasite Plasmodium vinckei revealed the transcriptional cascade commonly observed in malaria parasites. Microsampling is a quick, robust and cost-efficient approach to sample collection for in vivo time-series transcriptomic studies in rodent malaria parasites.
dc.publisherNCBI
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/bioproject/?term=PRJEB27301
dc.titleA fast and cost-effective microsampling protocol incorporating reduced animal usage for time-series transcriptomics in rodent malaria parasites
dc.typeBioproject
dc.typeDataset
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentPathogen Genomics Laboratory
dc.contributor.institutionMalaria Unit, Department of Pathology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.
kaust.personRamaprasad, Abhinay
kaust.personSubudhi, Amit
kaust.personPain, Arnab
dc.relation.issupplementtoDOI:10.1101/350546
dc.relation.issupplementtoDOI:10.1186/s12936-019-2659-4
display.relations<b>Is Supplement To:</b><br/> <ul><li><i>[Article]</i> <br/> Ramaprasad A, Subudhi AK, Culleton R, Pain A (2019) A fast and cost-effective microsampling protocol incorporating reduced animal usage for time-series transcriptomics in rodent malaria parasites. Malaria Journal 18. Available: http://dx.doi.org/10.1186/s12936-019-2659-4.. DOI: <a href="https://doi.org/10.1186/s12936-019-2659-4" >10.1186/s12936-019-2659-4</a> Handle: <a href="http://hdl.handle.net/10754/628424" >10754/628424</a></a></li></ul>
dc.identifier.bioprojectPRJEB27301


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