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dc.contributor.authorZeeshan, Mohammad
dc.contributor.authorPandey, Rajan
dc.contributor.authorSubudhi, Amit
dc.contributor.authorFerguson, David J P
dc.contributor.authorKaur, Gursimran
dc.contributor.authorRashpa, Ravish
dc.contributor.authorNugmanova, Raushan
dc.contributor.authorBrady, Declan
dc.contributor.authorBottrill, Andrew R.
dc.contributor.authorVaughan, Sue
dc.contributor.authorBrochet, Mathieu
dc.contributor.authorBollen, Mathieu
dc.contributor.authorPain, Arnab
dc.contributor.authorHolder, Anthony A.
dc.contributor.authorGuttery, David
dc.contributor.authorTewari, Rita
dc.date.accessioned2021-01-19T06:50:02Z
dc.date.available2021-01-19T06:50:02Z
dc.date.issued2021-01-17
dc.identifier.citationZeeshan, M., Pandey, R., Subudhi, A. K., Ferguson, D. J. P., Kaur, G., Rashpa, R., … Tewari, R. (2021). Protein Phosphatase 1 regulates atypical chromosome segregation and cell polarity during mitotic and meiotic division in Plasmodium sexual stages. doi:10.1101/2021.01.15.426883
dc.identifier.doi10.1101/2021.01.15.426883
dc.identifier.urihttp://hdl.handle.net/10754/666922
dc.description.abstractAbstractPP1 is a conserved eukaryotic serine/threonine phosphatase that regulates many aspects of mitosis and meiosis, often working in concert with other phosphatases, such as CDC14 and CDC25. The proliferative stages of the parasite life cycle include sexual development within the mosquito vector, with male gamete formation characterized by an atypical rapid mitosis, consisting of three rounds of DNA synthesis, successive spindle formation with clustered kinetochores, and a meiotic stage during zygote to ookinete development following fertilization. It is unclear how PP1 is involved in these unusual processes. Using real-time live-cell and ultrastructural imaging, conditional gene knockdown, RNA-seq and proteomic approaches, we show that Plasmodium PP1 is involved in both chromosome segregation during mitotic exit, and establishment of cell polarity during zygote development in the mosquito midgut, suggesting that small molecule inhibitors of PP1 should be explored for blocking parasite transmission.
dc.description.sponsorshipWe thank Julie Rodgers for helping to maintain the insectary and other technical works, and the personnel at the Bioscience Core Laboratory (BCL) in KAUST for sequencing the RNA samples and producing the raw datasets.
dc.publisherCold Spring Harbor Laboratory
dc.relation.urlhttp://biorxiv.org/lookup/doi/10.1101/2021.01.15.426883
dc.rightsArchived with thanks to Cold Spring Harbor Laboratory
dc.titleProtein Phosphatase 1 regulates atypical chromosome segregation and cell polarity during mitotic and meiotic division in Plasmodium sexual stages
dc.typePreprint
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience
dc.contributor.departmentBioscience Program
dc.eprint.versionPre-print
dc.contributor.institutionSchool of Life Sciences, University of Nottingham, Nottingham, UK.
dc.contributor.institutionNuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford, UK.
dc.contributor.institutionDepartment of Biological and Medical Sciences, Faculty of Health and Life Science, Oxford Brookes University, Gipsy Lane, Oxford, UK.
dc.contributor.institutionDepartment of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
dc.contributor.institutionSchool of Life Sciences, Gibbet Hill Campus, University of Warwick, Coventry CV47AL, UK.
dc.contributor.institutionLaboratory of Biosignaling and Therapeutics, KU Leuven Department of Cellular and Molecular Medicine, University of Leuven, B-3000 Leuven, Belgium.
dc.contributor.institutionResearch Center for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE); Hokkaido University, Sapporo, Japan.
dc.contributor.institutionMalaria Parasitology Laboratory, The Francis Crick Institute, London, UK.
dc.contributor.institutionLeicester Cancer Research Centre, University of Leicester, Leicester, UK .
kaust.personSubudhi, Amit
kaust.personNugmanova, Raushan
kaust.personPain, Arnab
refterms.dateFOA2021-01-19T06:50:49Z
kaust.acknowledged.supportUnitBioscience Core Laboratory (BCL)


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