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    Protein Phosphatase 1 regulates atypical chromosome segregation and cell polarity during mitotic and meiotic division in Plasmodium sexual stages

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    Preprintfile1.pdf
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    Type
    Preprint
    Authors
    Zeeshan, Mohammad cc
    Pandey, Rajan
    Subudhi, Amit cc
    Ferguson, David J P cc
    Kaur, Gursimran
    Rashpa, Ravish
    Nugmanova, Raushan
    Brady, Declan cc
    Bottrill, Andrew R. cc
    Vaughan, Sue cc
    Brochet, Mathieu cc
    Bollen, Mathieu cc
    Pain, Arnab cc
    Holder, Anthony A. cc
    Guttery, David cc
    Tewari, Rita cc
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience
    Bioscience Program
    Date
    2021-01-17
    Permanent link to this record
    http://hdl.handle.net/10754/666922
    
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    Abstract
    AbstractPP1 is a conserved eukaryotic serine/threonine phosphatase that regulates many aspects of mitosis and meiosis, often working in concert with other phosphatases, such as CDC14 and CDC25. The proliferative stages of the parasite life cycle include sexual development within the mosquito vector, with male gamete formation characterized by an atypical rapid mitosis, consisting of three rounds of DNA synthesis, successive spindle formation with clustered kinetochores, and a meiotic stage during zygote to ookinete development following fertilization. It is unclear how PP1 is involved in these unusual processes. Using real-time live-cell and ultrastructural imaging, conditional gene knockdown, RNA-seq and proteomic approaches, we show that Plasmodium PP1 is involved in both chromosome segregation during mitotic exit, and establishment of cell polarity during zygote development in the mosquito midgut, suggesting that small molecule inhibitors of PP1 should be explored for blocking parasite transmission.
    Citation
    Zeeshan, M., Pandey, R., Subudhi, A. K., Ferguson, D. J. P., Kaur, G., Rashpa, R., … Tewari, R. (2021). Protein Phosphatase 1 regulates atypical chromosome segregation and cell polarity during mitotic and meiotic division in Plasmodium sexual stages. doi:10.1101/2021.01.15.426883
    Sponsors
    We thank Julie Rodgers for helping to maintain the insectary and other technical works, and the personnel at the Bioscience Core Laboratory (BCL) in KAUST for sequencing the RNA samples and producing the raw datasets.
    Publisher
    Cold Spring Harbor Laboratory
    DOI
    10.1101/2021.01.15.426883
    Additional Links
    http://biorxiv.org/lookup/doi/10.1101/2021.01.15.426883
    ae974a485f413a2113503eed53cd6c53
    10.1101/2021.01.15.426883
    Scopus Count
    Collections
    Biological and Environmental Sciences and Engineering (BESE) Division; Preprints; Bioscience Program

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