Protein Phosphatase 1 regulates atypical chromosome segregation and cell polarity during mitotic and meiotic division in Plasmodium sexual stages
Type
PreprintAuthors
Zeeshan, Mohammad
Pandey, Rajan
Subudhi, Amit

Ferguson, David J P

Kaur, Gursimran
Rashpa, Ravish
Nugmanova, Raushan
Brady, Declan

Bottrill, Andrew R.

Vaughan, Sue

Brochet, Mathieu

Bollen, Mathieu

Pain, Arnab

Holder, Anthony A.

Guttery, David

Tewari, Rita

KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience
Bioscience Program
Date
2021-01-17Permanent link to this record
http://hdl.handle.net/10754/666922
Metadata
Show full item recordAbstract
AbstractPP1 is a conserved eukaryotic serine/threonine phosphatase that regulates many aspects of mitosis and meiosis, often working in concert with other phosphatases, such as CDC14 and CDC25. The proliferative stages of the parasite life cycle include sexual development within the mosquito vector, with male gamete formation characterized by an atypical rapid mitosis, consisting of three rounds of DNA synthesis, successive spindle formation with clustered kinetochores, and a meiotic stage during zygote to ookinete development following fertilization. It is unclear how PP1 is involved in these unusual processes. Using real-time live-cell and ultrastructural imaging, conditional gene knockdown, RNA-seq and proteomic approaches, we show that Plasmodium PP1 is involved in both chromosome segregation during mitotic exit, and establishment of cell polarity during zygote development in the mosquito midgut, suggesting that small molecule inhibitors of PP1 should be explored for blocking parasite transmission.Citation
Zeeshan, M., Pandey, R., Subudhi, A. K., Ferguson, D. J. P., Kaur, G., Rashpa, R., … Tewari, R. (2021). Protein Phosphatase 1 regulates atypical chromosome segregation and cell polarity during mitotic and meiotic division in Plasmodium sexual stages. doi:10.1101/2021.01.15.426883Sponsors
We thank Julie Rodgers for helping to maintain the insectary and other technical works, and the personnel at the Bioscience Core Laboratory (BCL) in KAUST for sequencing the RNA samples and producing the raw datasets.Publisher
Cold Spring Harbor LaboratoryAdditional Links
http://biorxiv.org/lookup/doi/10.1101/2021.01.15.426883ae974a485f413a2113503eed53cd6c53
10.1101/2021.01.15.426883