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dc.contributor.authorBi, Chongwei
dc.contributor.authorWang, Lin
dc.contributor.authorYuan, Baolei
dc.contributor.authorzhou, xuan
dc.contributor.authorHuang, Yanyi
dc.contributor.authorLi, Mo
dc.contributor.authorLi, Yu
dc.contributor.authorPang, Yuhong
dc.contributor.authorGao, Xin
dc.contributor.authorwang, sheng
dc.date.accessioned2021-01-17T10:39:38Z
dc.date.available2021-01-17T10:39:38Z
dc.date.issued2020-02-12
dc.identifier.urihttp://hdl.handle.net/10754/666916
dc.description.abstractWe develop a universal method to label individual DNA molecules for analyzing diverse types of rare genetic variants, with frequency as low as 4x10-5, using short- or long-read sequencing. It enables base-resolution haplotype-resolved quantitative characterization of rare variants. It provides the first quantitative evidence of persistent nonrandom large deletions and insertions following DNA repair of double-strand breaks induced by CRISPR-Cas9 in human pluripotent stem cells.
dc.publisherNCBI
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA606194
dc.titleIndividual-molecule Sequencing (IDMseq) of PANX1 gene in hESCs
dc.typeBioproject
dc.typeDataset
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentComputer Science
dc.contributor.departmentComputer Science Program
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.contributor.departmentLaboratory of Stem Cell and Regeneration, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia.
dc.contributor.departmentStructural and Functional Bioinformatics Group
dc.contributor.institutionPresent address: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, China.
dc.contributor.institutionBeijing Advanced Innovation Center for Genomics (ICG), Biomedical Pioneering Innovation Center (BIOPIC), School of Life Sciences, College of Chemistry, College of Engineering, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China.
dc.contributor.institutionInstitute for Cell Analysis, Shenzhen Bay Laboratory, Shenzhen, China.
kaust.personBi, Chongwei
kaust.personWang, Lin
kaust.personYuan, Baolei
kaust.personZhou, Xuan
kaust.personLi, Mo
kaust.personLi, Yu
kaust.personGao, Xin
kaust.personWang, Sheng
dc.relation.issupplementtoDOI:10.1186/s13059-020-02143-8
display.relations<b>Is Supplement To:</b><br/> <ul><li><i>[Article]</i> <br/> Bi, C., Wang, L., Yuan, B., Zhou, X., Li, Y., Wang, S., … Li, M. (2020). Long-read individual-molecule sequencing reveals CRISPR-induced genetic heterogeneity in human ESCs. Genome Biology, 21(1). doi:10.1186/s13059-020-02143-8. DOI: <a href="https://doi.org/10.1186/s13059-020-02143-8" >10.1186/s13059-020-02143-8</a> Handle: <a href="http://hdl.handle.net/10754/661565" >10754/661565</a></a></li></ul>
dc.identifier.bioprojectPRJNA606194


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