Type
BioprojectDataset
Authors
Bi, ChongweiWang, Lin
Yuan, Baolei
Zhou, Xuan
Huang, Yanyi
Li, Mo

Li, Yu
Pang, Yuhong
Gao, Xin

wang, sheng
KAUST Department
Bioscience ProgramBiological and Environmental Sciences and Engineering (BESE) Division
Laboratory of Stem Cell and Regeneration, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia.
Computer Science Program
Computer Science
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Computational Bioscience Research Center (CBRC)
Date
2020-02-12Permanent link to this record
http://hdl.handle.net/10754/666916
Metadata
Show full item recordAbstract
We develop a universal method to label individual DNA molecules for analyzing diverse types of rare genetic variants, with frequency as low as 4x10-5, using short- or long-read sequencing. It enables base-resolution haplotype-resolved quantitative characterization of rare variants. It provides the first quantitative evidence of persistent nonrandom large deletions and insertions following DNA repair of double-strand breaks induced by CRISPR-Cas9 in human pluripotent stem cells.Publisher
NCBIAdditional Links
https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA606194Relations
Is Supplement To:- [Article]
Bi, C., Wang, L., Yuan, B., Zhou, X., Li, Y., Wang, S., … Li, M. (2020). Long-read individual-molecule sequencing reveals CRISPR-induced genetic heterogeneity in human ESCs. Genome Biology, 21(1). doi:10.1186/s13059-020-02143-8. DOI: 10.1186/s13059-020-02143-8 Handle: 10754/661565