Individual-molecule Sequencing (IDMseq) of PANX1 gene in hESCs

Type
Bioproject
Dataset
Authors
Bi, Chongwei
Wang, Lin
Yuan, Baolei
Zhou, Xuan
Huang, Yanyi
Li, Mo cc
Li, Yu
Pang, Yuhong
Gao, Xin cc
wang, sheng
KAUST Department
Bioscience Program
Biological and Environmental Sciences and Engineering (BESE) Division
Laboratory of Stem Cell and Regeneration, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia.
Computer Science Program
Computer Science
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Computational Bioscience Research Center (CBRC)
Date
2020-02-12
Permanent link to this record
http://hdl.handle.net/10754/666916

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Abstract
We develop a universal method to label individual DNA molecules for analyzing diverse types of rare genetic variants, with frequency as low as 4x10-5, using short- or long-read sequencing. It enables base-resolution haplotype-resolved quantitative characterization of rare variants. It provides the first quantitative evidence of persistent nonrandom large deletions and insertions following DNA repair of double-strand breaks induced by CRISPR-Cas9 in human pluripotent stem cells.
Publisher
NCBI
Additional Links
https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA606194
Relations
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  • [Article]
    Bi, C., Wang, L., Yuan, B., Zhou, X., Li, Y., Wang, S., … Li, M. (2020). Long-read individual-molecule sequencing reveals CRISPR-induced genetic heterogeneity in human ESCs. Genome Biology, 21(1). doi:10.1186/s13059-020-02143-8. DOI: 10.1186/s13059-020-02143-8 Handle: 10754/661565
Collections
Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Computer Science Program; Computational Bioscience Research Center (CBRC); Datasets; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division