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dc.contributor.authorRayapuram, Naganand
dc.contributor.authorJarad, Mai
dc.contributor.authorAlhoraibi, Hanna
dc.contributor.authorBigeard, Jean
dc.contributor.authorAbulfaraj, Aala A.
dc.contributor.authorVolz, Ronny
dc.contributor.authorMariappan, Kiruthiga
dc.contributor.authorAlmeida-Trapp, Marilia
dc.contributor.authorSchlöffel, Maria
dc.contributor.authorLastrucci, Emmanuelle
dc.contributor.authorBonhomme, Ludovic
dc.contributor.authorGust, Andrea A.
dc.contributor.authorMithöfer, Axel
dc.contributor.authorArold, Stefan T.
dc.contributor.authorPflieger, Delphine
dc.contributor.authorHirt, Heribert
dc.date.accessioned2021-01-11T13:52:36Z
dc.date.available2021-01-11T13:52:36Z
dc.date.issued2021-01-08
dc.identifier.citationRayapuram, N., Jarad, M., Alhoraibi, H. M., Bigeard, J., Abulfaraj, A. A., Völz, R., … Hirt, H. (2021). Chromatin phosphoproteomics unravels a function for AT-hook motif nuclear localized protein AHL13 in PAMP-triggered immunity. Proceedings of the National Academy of Sciences, 118(3), e2004670118. doi:10.1073/pnas.2004670118
dc.identifier.issn0027-8424
dc.identifier.issn1091-6490
dc.identifier.pmid33419940
dc.identifier.doi10.1073/pnas.2004670118
dc.identifier.urihttp://hdl.handle.net/10754/666869
dc.description.abstractIn many eukaryotic systems during immune responses, mitogen-activated protein kinases (MAPKs) link cytoplasmic signaling to chromatin events by targeting transcription factors, chromatin remodeling complexes, and the RNA polymerase machinery. So far, knowledge on these events is scarce in plants and no attempts have been made to focus on phosphorylation events of chromatin-associated proteins. Here we carried out chromatin phosphoproteomics upon elicitor-induced activation of Arabidopsis. The events in WT were compared with those in mpk3, mpk4, and mpk6 mutant plants to decipher specific MAPK targets. Our study highlights distinct signaling networks involving MPK3, MPK4, and MPK6 in chromatin organization and modification, as well as in RNA transcription and processing. Among the chromatin targets, we characterized the AT-hook motif containing nuclear localized (AHL) DNA-binding protein AHL13 as a substrate of immune MAPKs. AHL13 knockout mutant plants are compromised in pathogen-associated molecular pattern (PAMP)-induced reactive oxygen species production, expression of defense genes, and PAMP-triggered immunity. Transcriptome analysis revealed that AHL13 regulates key factors of jasmonic acid biosynthesis and signaling and affects immunity toward Pseudomonas syringae and Botrytis cinerea pathogens. Mutational analysis of the phosphorylation sites of AHL13 demonstrated that phosphorylation regulates AHL13 protein stability and thereby its immune functions.
dc.description.sponsorshipWe thank Véronique Legros, Huma Khurram, and Huoming Zhang for technical assistance in mass spectometry; Stéphanie Pateyron and Alexandra Avon for technical support in molecular biology; and Jean Colcombet for kindly providing the constitutively active MAPK vectors. This work was supported by Agence Nationale de la Recherche ANR-2010-JCJC-1608 and ANR-14-CE19-0014 (to D.P.); Investissement d’Avenir Infrastructures Nationales en Biologie et Santé program (ProFI project, ANR10-INBS-08); and by King Abdullah University of Science and Technology BAS/1/1062-01-01 (to H.H.). The Institute of Plant Sciences Paris-Saclay benefits from the support of the LabEx Saclay Plant Sciences (ANR-10-LABX-0040-SPS). M.A.-T. was supported by a Humboldt-Capes fellowship.
dc.publisherProceedings of the National Academy of Sciences
dc.relation.urlhttp://www.pnas.org/lookup/doi/10.1073/pnas.2004670118
dc.rightsArchived with thanks to Proceedings of the National Academy of Sciences
dc.titleChromatin phosphoproteomics unravels a function for AT-hook motif nuclear localized protein AHL13 in PAMP-triggered immunity
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentComputational Bioscience Research Center, Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentHirt Lab
dc.contributor.departmentPlant Science
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.contributor.departmentStructural Biology and Engineering
dc.identifier.journalProceedings of the National Academy of Sciences
dc.rights.embargodate2021-07-08
dc.eprint.versionPost-print
dc.contributor.institutionCentre for Research in Agricultural Genomics, Consejo Superior de Investigaciones Cientificas, Catalan Institute for Food and Agricultural Research and Technology, Autonomous University of Barcelona, University of Barcelona, 08007 Barcelona, Spain.
dc.contributor.institutionDepartment of Biochemistry, Faculty of Science, King Abdulaziz University, 21551 Jeddah, Saudi Arabia.
dc.contributor.institutionCNRS, Institut National de Recherche pour l’Agriculture, l’Alimentation, et l’Environnement, University Evry, Institute of Plant Sciences Paris-Saclay, Université Paris-Saclay, 91405 Orsay, France.
dc.contributor.institutionInstitut National de Recherche pour l’Agriculture, l’Alimentation, et l’Environnement, Institute of Plant Sciences Paris-Saclay, Université de Paris, CNRS, 91405 Orsay, France.
dc.contributor.institutionDepartment of Biological Sciences, College of Sciences and Arts-Rabigh Campus, King Abdulaziz University, 21589 Jeddah, Saudi Arabia.
dc.contributor.institutionPlant Immunity Research Center, Seoul National University, 08826 Seoul, Korea.
dc.contributor.institutionResearch Group Plant Defense Physiology, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.
dc.contributor.institutionCenter for Plant Molecular Biology, Eberhard-Karls-Universität Tübingen, 72074 Tübingen, Germany.
dc.contributor.institutionCommissariat à l’Energie Atomique, INSERM, Interdisciplinary Research Institute Grenoble, Large Scale Biology Laboratory, University Grenoble Alpes, 38000 Grenoble, France.
dc.contributor.institutionInterdisciplinary Research Institute of Grenoble, Large Scale Biology Laboratory, CNRS, F-38000 Grenoble, France.
dc.contributor.institutionCNRS, LAMBE UMR 8587, Université d’Evry Val d’Essonne, 91000 Evry, France.
dc.contributor.institutionMax Perutz Laboratories, University of Vienna, 1030 Vienna, Austria.
dc.identifier.volume118
dc.identifier.issue3
dc.identifier.pagese2004670118
kaust.personRayapuram, Naganand
kaust.personJarad, Mai
kaust.personMariappan, Kiruthiga
kaust.personAlmeida-Trapp, Marilia
kaust.personArold, Stefan T.
kaust.personPflieger, Delphine
kaust.personHirt, Heribert
kaust.grant.numberBAS/1/1062-01-01
dc.relation.issupplementedbybioproject:PRJNA489287
display.relations<b>Is Supplemented By:</b><br/> <ul><li><i>[Bioproject]</i> <br/> Title: Chromatin phosphoproteomics unravels a function for AT-hook motif nuclear localized protein AHL13 in PAMP-triggered immunity. Publication Date: 2021-03-22. bioproject: <a href="https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA489287" >PRJNA489287</a> Handle: <a href="http://hdl.handle.net/10754/668249" >10754/668249</a></a></li></ul>


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