Metabolomic and Biochemical Analysis of Two Potato (Solanum tuberosum L.) Cultivars Exposed to In Vitro Osmotic and Salt Stresses.
Type
ArticleKAUST Department
Water Desalination and Reuse Research Center (WDRC)Biological and Environmental Sciences and Engineering (BESE) Division
Date
2021-01-09Submitted Date
2020-12-16Permanent link to this record
http://hdl.handle.net/10754/666867
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Globally, many crop production areas are threatened by drought and salinity. Potato ($\textit{Solanum tuberosum}$ L.) is susceptible to these challenging environmental conditions. In this study, an in vitro approach was employed to compare the tolerance of potato cultivars 'BARI-401' (red skin) and 'Spunta' (yellow skin). To simulate ionic and osmotic stress, MS media was supplemented with lithium chloride (LiCl 20 mM) and mannitol (150 mM). GC-MS and spectrophotometry techniques were used to determine metabolite accumulation. Other biochemical properties, such as total phenols concentration (TPC), total flavonoids concentration (TFC), antioxidant capacity (DPPH free radical scavenging capacity), polyphenol oxidase (PPO), and peroxidase (POD) activities, were also measured. The two cultivars respond differently to ionic and osmotic stress treatments, with Spunta accumulating more defensive metabolites in response, indicating a higher level of tolerance. While further investigation of the physiological and biochemical responses of these varieties to drought and salinity is required, the approach taken in this paper provides useful information prior to open field evaluation.Citation
Hamooh, B. T., Sattar, F. A., Wellman, G., & Mousa, M. A. A. (2021). Metabolomic and Biochemical Analysis of Two Potato (Solanum tuberosum L.) Cultivars Exposed to In Vitro Osmotic and Salt Stresses. Plants, 10(1), 98. doi:10.3390/plants10010098Sponsors
The authors are grateful to the Deanship of Graduate Studies (DGS), king Abdulaziz University, for endowment of the Ph.D. scholarship under student ID. No 1801819. Moreover, the authors acknowledge the moral and technical support from the Department of Arid Land Agriculture.Publisher
MDPI AGJournal
Plants (Basel, Switzerland)PubMed ID
33418964Additional Links
https://www.mdpi.com/2223-7747/10/1/98ae974a485f413a2113503eed53cd6c53
10.3390/plants10010098
Scopus Count
Except where otherwise noted, this item's license is described as This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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