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dc.contributor.authorMelino, Vanessa Jane
dc.contributor.authorPlett, D C
dc.contributor.authorBendre, P
dc.contributor.authorThomsen, H C
dc.contributor.authorZeisler-Diehl, V V
dc.contributor.authorSchreiber, L
dc.contributor.authorKronzucker, H J
dc.date.accessioned2021-01-04T10:31:21Z
dc.date.available2021-01-04T10:31:21Z
dc.date.issued2020-12-17
dc.date.submitted2020-11-03
dc.identifier.citationMelino, V. J., Plett, D. C., Bendre, P., Thomsen, H. C., Zeisler-Diehl, V. V., Schreiber, L., & Kronzucker, H. J. (2021). Nitrogen depletion enhances endodermal suberization without restricting transporter-mediated root NO3- influx. Journal of Plant Physiology, 257, 153334. doi:10.1016/j.jplph.2020.153334
dc.identifier.issn0176-1617
dc.identifier.pmid33373827
dc.identifier.doi10.1016/j.jplph.2020.153334
dc.identifier.urihttp://hdl.handle.net/10754/666802
dc.description.abstractRoots vary their permeability to aid radial transport of solutes towards xylem vessels in response to nutritional cues. Nitrogen (N) depletion was previously shown to induce early suberization of endodermal cell walls and reduce hydraulic conductivity of barley roots suggesting reduced apoplastic transport of ions (Armand et al., 2019). Suberization may also limit transcellular ion movement by blocking access to transporters (Barberon et al., 2016). The aim of this study was to confirm that N depletion induced suberization in the roots of barley and demonstrate that this was a specific effect in response to NO$_{3}$$^{-}$ depletion. Furthermore, in roots with early and enhanced suberization, we assessed their ability for transporter-mediated NO$_{3}$$^{-}$ influx. N depletion induced lateral root elongation and early and enhanced endodermal suberization of the seminal root of each genotype. Both root to shoot NO$_{3}$$^{-}$ translocation and net N uptake was half that of plants supplied with steady-state NO$_{3}$$^{-}$. Genes with predicted functions in suberin synthesis (HvHORST) and NO$_{3}$$^{-}$ transport (HvNRT2.2) were induced under N-deplete conditions. N-deplete roots had a higher capacity for high-affinity NO$_{3}$- influx in early suberized roots than under optimal NO$_{3}$$^{-}$. In conclusion, NO$_{3}$$^{-}$ depletion induced early and enhanced suberization in the roots of barley, however, suberization did not restrict transcellular NO$_{3}$$^{-}$ transport.
dc.description.sponsorshipThe authors would like to acknowledge the University of Melbourne, School of Agriculture and Food research investment fund, Australia. We acknowledge the Melbourne Histology and Histopathology platform and expert advice of Laura Leone and the University of Melbourne Biological Optical Microscopy (BOMP) platform. We would like to acknowledge Melbourne TrACEES Platform for the service and expert advice by Michael Hall.
dc.publisherElsevier BV
dc.relation.urlhttps://linkinghub.elsevier.com/retrieve/pii/S0176161720302248
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Journal of plant physiology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of plant physiology, [257, , (2020-12-29)] DOI: 10.1016/j.jplph.2020.153334 . © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleNitrogen depletion enhances endodermal suberization without restricting transporter-mediated root NO3- influx
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentDivision of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia.
dc.identifier.journalJournal of plant physiology
dc.rights.embargodate2022-12-26
dc.eprint.versionPost-print
dc.contributor.institutionSchool of Agriculture and Food, The University of Melbourne, Melbourne, VIC, 3010 Australia
dc.contributor.institutionSchool of Agriculture, Food and Wine, The University of Adelaide, Urrbrae, SA, 5064 Australia
dc.contributor.institutionCarlsberg Research Laboratory, J.C. Jacobsens Gade 4, 1799, Copenhagen V, Denmark
dc.contributor.institutionDepartment of Ecophysiology, Institute of Cellular and Molecular Botany, University of Bonn, 53115, Bonn, Germany
dc.contributor.institutionFaculty of Land and Food Systems, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada
dc.identifier.volume257
dc.identifier.pages153334
kaust.personMelino, Vanessa Jane
dc.date.accepted2020-11-21
dc.identifier.eid2-s2.0-85098197081
dc.date.published-online2020-12-17
dc.date.published-print2021-02


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