Show simple item record

dc.contributor.authorAlQahtani, Manal Faisal
dc.contributor.authorBajracharya, Suman
dc.contributor.authorKaturi, Krishna
dc.contributor.authorAli, Muhammad
dc.contributor.authorRagab, Alaa I.
dc.contributor.authorMichoud, Gregoire
dc.contributor.authorDaffonchio, Daniele
dc.contributor.authorSaikaly, Pascal
dc.date.accessioned2020-12-28T13:12:41Z
dc.date.available2020-12-28T13:12:41Z
dc.date.issued2019-11-27
dc.identifier.urihttp://hdl.handle.net/10754/666722
dc.description.abstractHomoacetogens are efficient CO2 fixing bacteria using H2 as electron donor to produce acetate. These organisms can be enriched at the biocathode of microbial electrosynthesis (MES) for electricity driven CO2 reduction to acetate. Studies exploring homoacetogens in MES are mainly conducted using pure or mix-culture anaerobic inocula from samples with standard environmental conditions. Extreme marine environments host unique microbial communities including homoacetogens that may have unique capabilities due to their adaptation to harsh environmental conditions. Anaerobic deep-sea brine pools are hypersaline and metalliferous environments and homoacetogens can be expected to live in these environments due to their remarkable metabolic flexibility and energy efficient biosynthesis. However, brine pools have never been explored as inocula for the enrichment of homacetogens in MES. Here we used the saline water from a Red Sea brine pool as inoculum for the enrichment of halophilic homoacetogens at the biocathode of MES. Volatile fatty acids, especially acetate, along with hydrogen gas were produced in MES systems operated at 25 and 10 percent salinity. Acetate concentration increased when MES was operated at a lower salinity 3.5 percent, representing typical seawater salinity. Amplicon sequencing and genome-centric metagenomics of matured cathodic biofilm showed the dominance of the genus Marinobacter and phylum Firmicutes at all tested salinities. Seventeen high quality draft metagenome assembled genomes (MAGs) were extracted from the biocathode samples. The recovered MAGs accounted for 87 percent of the quality filtered sequence reads. Genome analysis of the MAGs suggested CO2 fixation via Wood-Ljundahl pathway by members of the phylum Firmicutes and the fixed CO2 was possibly utilized by Marinobacter sp. for growth by consuming O2 escaping from the anode to the cathode for respiration. The enrichment of Marinobacter sp. with homoacetogens was only possible because of the specific cathodic environment in MES. These findings suggest that in organic carbon limited saline environments, Marinobacter spp. can live in consortia with CO2 fixing bacteria such as homoacetogens, which can provide them with fixed carbon as a source of carbon and energy
dc.publisherNCBI
dc.relation.haspartbiosample:SAMN11897331
dc.relation.haspartbiosample:SAMN11897271
dc.relation.haspartbiosample:SAMN11897272
dc.relation.haspartbiosample:SAMN11897273
dc.relation.haspartbiosample:SAMN11897274
dc.relation.haspartbiosample:SAMN11897275
dc.relation.haspartbiosample:SAMN11897276
dc.relation.haspartbiosample:SAMN11897277
dc.relation.haspartbiosample:SAMN11897278
dc.relation.haspartbiosample:SAMN11897279
dc.relation.haspartbiosample:SAMN11897280
dc.relation.haspartbiosample:SAMN11897281
dc.relation.haspartbiosample:SAMN11897282
dc.relation.haspartbiosample:SAMN11897283
dc.relation.haspartbiosample:SAMN11897284
dc.relation.haspartbiosample:SAMN11897285
dc.relation.haspartbiosample:SAMN11897326
dc.relation.haspartbiosample:SAMN11897327
dc.relation.haspartbiosample:SAMN11897328
dc.relation.haspartbiosample:SAMN11897329
dc.relation.haspartbiosample:SAMN11897330
dc.relation.haspartbiosample:SAMN11897332
dc.relation.haspartbiosample:SAMN11897333
dc.relation.haspartbiosample:SAMN11897334
dc.relation.haspartbiosample:SAMN11897335
dc.relation.haspartbiosample:SAMN11897336
dc.relation.haspartbiosample:SAMN11897337
dc.relation.haspartbiosample:SAMN11897338
dc.relation.haspartbiosample:SAMN11897339
dc.relation.haspartbiosample:SAMN11897340
dc.relation.haspartbiosample:SAMN11897341
dc.relation.haspartbiosample:SAMN11897342
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA545216
dc.titleEnrichment of Marinobacter sp. and Halophilic Homoacetogens at the Biocathode of Microbial Electrosynthesis System Inoculated With Red Sea Brine Pool
dc.typeBioproject
dc.typeDataset
dc.contributor.departmentEnvironmental Science and Engineering Program
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)
dc.contributor.departmentKing Abdullah University of Science and Technology, Water Desalination and Reuse Center, Biological and Environmental Science and Engineering Division, Thuwal, Saudi Arabia
dc.contributor.departmentBioscience Program
kaust.personAlQahtani, Manal Faisal
kaust.personBajracharya, Suman
kaust.personKaturi, Krishna
kaust.personAli, Muhammad
kaust.personRagab, Alaa I.
kaust.personMichoud, Gregoire
kaust.personDaffonchio, Daniele
kaust.personSaikaly, Pascal
dwc.taxon.scientificNamemarine metagenome
dc.relation.issupplementtoDOI:10.3389/fmicb.2019.02563
display.relations<b>Is Supplement To:</b><br/> <ul><li><i>[Article]</i> <br/> Alqahtani, M. F., Bajracharya, S., Katuri, K. P., Ali, M., Ragab, A., Michoud, G., … Saikaly, P. E. (2019). Enrichment of Marinobacter sp. and Halophilic Homoacetogens at the Biocathode of Microbial Electrosynthesis System Inoculated With Red Sea Brine Pool. Frontiers in Microbiology, 10. doi:10.3389/fmicb.2019.02563. DOI: <a href="https://doi.org/10.3389/fmicb.2019.02563" >10.3389/fmicb.2019.02563</a> Handle: <a href="http://hdl.handle.net/10754/660090" >10754/660090</a></a></li></ul>
dc.identifier.bioprojectPRJNA545216


This item appears in the following Collection(s)

Show simple item record