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dc.contributor.authorNaeem, Raeece
dc.contributor.authorPain, Arnab
dc.date.accessioned2020-12-23T11:36:12Z
dc.date.available2020-12-23T11:36:12Z
dc.date.issued2020-12-22
dc.identifier.citationMohamed Ghazzali, R. N., & Pain, A. (2020). assayM: a web application to monitor mutations in COVID-19 diagnostic assays. doi:10.1101/2020.12.18.423467
dc.identifier.doi10.1101/2020.12.18.423467
dc.identifier.urihttp://hdl.handle.net/10754/666618
dc.description.abstractReverse Transcriptase - Polymerase Chain Reaction (RT-PCR) is the gold standard as diagnostic assays for the detection of COVID-19 and the specificity and sensitivity of these assays depend on the complementarity of the RT-PCR primers to the genome of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Since the virus mutates over time during replication cycles, there is an urgent need to continuously monitor the virus genome for appearances of mutations and mismatches in the PCR primers used in these assays. Here we present assayM, a web application to explore and monitor mutations introduced in the primer and probe sequences published by the World Health Organisation (WHO) or in any custom-designed assay primers for SARS-CoV-2 detection assays in globally available SARS-CoV-2 genome datasets.
dc.description.sponsorshipThis work has been supported by the KAUST Rapid Research Response Team (R3T) funding and the BAS/01-01-1020 fund to Arnab Pain.
dc.publisherCold Spring Harbor Laboratory
dc.relation.urlhttp://biorxiv.org/lookup/doi/10.1101/2020.12.18.423467
dc.rightsArchived with thanks to Cold Spring Harbor Laboratory
dc.titleassayM: a web application to monitor mutations in COVID-19 diagnostic assays.
dc.typePreprint
dc.contributor.departmentPathogen Genomics Laboratory, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia.
dc.contributor.departmentBioscience Program
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.eprint.versionPre-print
dc.contributor.institutionResearch Center for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE); Hokkaido University, N20 W10 Kita-ku, Sapporo, 001-0020 Japan.
kaust.personMohamed Ghazzali, Raeece Naeem
kaust.personPain, Arnab
refterms.dateFOA2020-12-23T11:36:50Z


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