Comparative genomic and transcriptome analysis of M. kansasii subtypes provides new insights on its taxonomy and pathogenicity
Ben Rached, Fathia
Amini, Mohammad S
Adroub, Sabir A
Van Ingen, Jakko
Abdallah, Abdallah M
KAUST DepartmentBioscience Program
Biological and Environmental Sciences and Engineering (BESE) Division
Pathogen Genomics Laboratory, BESE Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.
Permanent link to this recordhttp://hdl.handle.net/10754/666535
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AbstractMycobacterium kansasii shows a close phylogenetic relationship with Mycobacterium tuberculosis which causes more than 1.5 million deaths per year in recent years. Seven subtypes (I-VII) have been identified using molecular biology approach while Type I-V were the most commonly discovered, and subtype I represents the most frequent isolates contributing to human disease. To investigate the genotypes and pathogenicity components of M. kansasii, we have sequenced the genome of M. kansasii subtypes KAUST I-V with PacBio sequencing technique to describe complete and accurate genomes, then undertook comparative genomics and a transcriptomic approach. We proposed that the “subtypes” of M. kansasii should be considered as different species and developed the genotyping primers for Type I-V. We have also found out several potential virulence factors and determined the pathogenic role of the espACD operon in M. kansasii.
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Guan, Q., Ummels, R., Ben-Rached, F., Alzahid, Y., Amini, M. S., Adroub, S. A., … Pain, A. (2020). Comparative Genomic and Transcriptomic Analyses of Mycobacterium kansasii Subtypes Provide New Insights Into Their Pathogenicity and Taxonomy. Frontiers in Cellular and Infection Microbiology, 10. doi:10.3389/fcimb.2020.00122. DOI: 10.3389/fcimb.2020.00122 Handle: 10754/662501