Self-assembling tetrameric peptides allow in situ 3D bioprinting under physiological conditions

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Rauf et al. Accepted manuscript J Mater Chem B.PDF
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Accepted Manuscript
Embargo End Date:
2021-12-17
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Type
Article
Authors
Rauf, Sakandar cc
Susapto, Hepi Hari cc
Kahin, Kowther
Alshehi, Salwa
Abdelrahman, Sherin
Lam, Jordy Homing
Asad, Sultan
Jadhav, Sandip
Sundaramurthi, Dhakshinamoorthy
Gao, Xin cc
Hauser, Charlotte cc
KAUST Department
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Bioscience Program
Biological and Environmental Sciences and Engineering (BESE) Division
Laboratory for Nanomedicine, Biological and Environmental Science and Engineering (BESE) Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
Computational Bioscience Research Center (CBRC), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
Computer Science Program
Computational Bioscience Research Center (CBRC)
Date
2020
Embargo End Date
2021-12-17
Permanent link to this record
http://hdl.handle.net/10754/666459

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Abstract
We have developed an in situ bioprinting method that allows printing of cells under true physiological conditions applying self-assembling ultrashort peptides as bioinks. This method avoids cell stressing methods such as UV-treatment, chemical crosslinking and viscous bioinks printing methods. We further demonstrate that different nanomaterials can easily be synthesized or incorporated in the 3D bioprinted peptide scaffolds which open up the possibility of functionalized 3D scaffolds.
Citation
Rauf, S., Susapto, H. H., Kahin, K., Alshehri, S., Abdelrahman, S., Lam, J. H., … Hauser, C. A. E. (2021). Self-assembling tetrameric peptides allow in situ 3D bioprinting under physiological conditions. Journal of Materials Chemistry B. doi:10.1039/d0tb02424d
Sponsors
The work was supported by funding from King Abdullah University of Science and Technology (KAUST). Synthesis of IVFK and IVZK peptides was first performed at the Institute of Bioengineering and Nanotechnology, A*Star, Singapore. The authors would like to acknowledge Prof. Takashi Gojobori, Hajime Ohyanagi and Dr. Katsuhiko Mineta for providing help in analyzing the RNA sequencing data. Visiting student and scholarship holder from the Studienstiftung des deutschen Volkes Ms. Annemarie Kehl, Georg-August University Goettingen, Germany, kindly measured the CD spectra. The authors would like to acknowledge KAUST’s Core Labs for supporting the sequencing and microscopy analysis.
Publisher
Royal Society of Chemistry (RSC)
Journal
Accepted by Journal of Materials Chemistry B
DOI
10.1039/d0tb02424d
Collections
Articles; Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program; Computer Science Program; Computational Bioscience Research Center (CBRC); Computer, Electrical and Mathematical Science and Engineering (CEMSE) Division