Type
BioprojectDataset
Authors
Abdallah, AbdallahWeerdenburg, Eveline
Guan, Qingtian

Ummels, Roy
Borggreve, S
Adroub, Sabir
Naeem, Raeece

Zhang, Huoming

Otto, Thomas
Bitter, Wilbert
Pain, Arnab

Malas, Tareq Majed Yasin

KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Bioscience Core Lab
Computer Science Program
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Date
2015-09-01Permanent link to this record
http://hdl.handle.net/10754/666454
Metadata
Show full item recordAbstract
Although the Bacillus Calmette-Guérin (BCG) vaccines against tuberculosis have been available for more than 90 years, their effectiveness has been hindered by variable protective efficacy and a lack of lasting memory responses. One factor in this variability may be the diversity of the BCG strains that are used around the world, particularly the changes in the genomic material and the resulting differences in gene expression. We have compared the genomes and transcriptomes of a global collection of fourteen of the most widely used BCG strains at single base-pair resolution. We have also used quantitative proteomics to identify key differences in expression at the protein level across five representative BCG strains of the four tandem duplication groups. We provide a comprehensive map of single nucleotide polymorphisms (SNPs), copy number variation and indels across fourteen BCG strains. The distribution of SNPs across the BCG lineages allowed clustering of these strains to generate a linear phylogeny and refining the previous genealogies of BCG strains. Transcriptional and proteomic profiling revealed a metabolic remodeling in BCG strains that may reflect in altered immunogenicity and possibly vaccine efficacy. Together, the presented datasets represent the most comprehensive catalogue of genomic variation across a global collection of BCG strains.Publisher
NCBIAdditional Links
https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJEB8560Relations
Is Supplement To:- [Preprint]
Abdallah AM, Weerdenburg E, Guan Q, Ummels R, Borggreve S, et al. (2018) WhiB6 is required for the secretion-dependent regulation of ESX-1 substrates in pathogenic mycobacteria. Available: http://dx.doi.org/10.1101/297440.. DOI: 10.1101/297440 Handle: 10754/628004 - [Article]
Abdallah AM, Weerdenburg EM, Guan Q, Ummels R, Borggreve S, et al. (2019) Integrated transcriptomic and proteomic analysis of pathogenic mycobacteria and their esx-1 mutants reveal secretion-dependent regulation of ESX-1 substrates and WhiB6 as a transcriptional regulator. PLOS ONE 14: e0211003. Available: http://dx.doi.org/10.1371/journal.pone.0211003.. DOI: 10.1371/journal.pone.0211003 Handle: 10754/631047