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dc.contributor.authorWang, Changzhi
dc.contributor.authorHong, Pei-Ying
dc.date.accessioned2020-12-13T13:44:21Z
dc.date.available2020-12-13T13:44:21Z
dc.date.issued2020-12-10
dc.date.submitted2020-10-19
dc.identifier.citationWang, C., & Hong, P.-Y. (2020). Genome-Resolved Metagenomics and Antibiotic Resistance Genes Analysis in Reclaimed Water Distribution Systems. Water, 12(12), 3477. doi:10.3390/w12123477
dc.identifier.issn2073-4441
dc.identifier.doi10.3390/w12123477
dc.identifier.urihttp://hdl.handle.net/10754/666343
dc.description.abstractWater reuse is increasingly pursued to alleviate global water scarcity. However, the wastewater treatment process does not achieve full removal of biological contaminants from wastewater, hence microorganisms and their genetic elements can be disseminated into the reclaimed water distribution systems (RWDS). In this study, reclaimed water samples are investigated via metagenomics to assess their bacterial diversity, metagenome-assembled genomes (MAGs) and antibiotic resistance genes (ARGs) at both point of entry (POE) and point of use (POU) in 3 RWDS. The number of shared bacterial orders identified by metagenome was higher at the POE than POU among the three sites, indicating that specific conditions in RWDS can cause further differentiation in the microbial communities at the end of the distribution system. Two bacterial orders, namely Rhizobiales and Sphingomonadales, had high replication rates in two of the examined RWDS (i.e., site A and B), and were present in higher relative abundance in POU than at POE. In addition, MAG and ARG relative abundance exhibited a strong correlation (R2 = 0.58) in POU, indicating that bacteria present in POU may have a high incidence of ARG. Specifically, resistance genes associated with efflux pump mechanisms (e.g., adeF and qacH) increased in its relative abundance from POU to POE at two of the RWDS (i.e., site A and B). When correlated with the water quality data that suggests a significantly lower dissolved organic carbon (DOC) concentration at site D than the other two RWDS, the metagenomic data suggest that low DOC is needed to maintain the biological stability of reclaimed water along the distribution network.
dc.description.sponsorshipThis research was funded by KAUST baseline funding BAS/1/1033-01-01 awarded to P.-Y. Hong.
dc.description.sponsorshipThe authors would like to acknowledge Amy Pruden and Emily Garner for providing the datasets and additional information pertaining to the samples.
dc.publisherMDPI AG
dc.relation.urlhttps://www.mdpi.com/2073-4441/12/12/3477
dc.rightsThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleGenome-Resolved Metagenomics and Antibiotic Resistance Genes Analysis in Reclaimed Water Distribution Systems
dc.typeArticle
dc.contributor.departmentEnvironmental Science and Engineering
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentEnvironmental Science and Engineering Program
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)
dc.identifier.journalWater
dc.eprint.versionPublisher's Version/PDF
dc.identifier.volume12
dc.identifier.issue12
dc.identifier.pages3477
kaust.personWang, Changzhi
kaust.personHong, Pei-Ying
kaust.grant.numberBAS/1/1033-01-01
dc.date.accepted2020-12-08
refterms.dateFOA2020-12-13T13:45:19Z
kaust.acknowledged.supportUnitKAUST baseline funding


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This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
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