GCN5 modulates salicylic acid homeostasis by regulating H3K14ac levels at the 5' and 3' ends of its target genes.
Type
ArticleAuthors
Kim, SoonKapPiquerez, Sophie J M
Ramirez-Prado, Juan S
Mastorakis, Emmanouil
Veluchamy, Alaguraj

Latrasse, David
Manza-Mianza, Deborah
Brik-Chaouche, Rim
Huang, Ying
Rodriguez-Granados, Natalia Y
Concia, Lorenzo
Blein, Thomas
Citerne, Sylvie
Bendahmane, Abdelhafid
Bergounioux, Catherine
Crespi, Martin
Mahfouz, Magdy M.

Raynaud, Cécile
Hirt, Heribert

Ntoukakis, Vardis

Benhamed, Moussa
KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionPlant Science
Desert Agriculture Initiative
Date
2020-05-13Submitted Date
2019-10-16Permanent link to this record
http://hdl.handle.net/10754/666299
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Show full item recordAbstract
The modification of histones by acetyl groups has a key role in the regulation of chromatin structure and transcription. The Arabidopsis thaliana histone acetyltransferase GCN5 regulates histone modifications as part of the Spt-Ada-Gcn5 Acetyltransferase (SAGA) transcriptional coactivator complex. GCN5 was previously shown to acetylate lysine 14 of histone 3 (H3K14ac) in the promoter regions of its target genes even though GCN5 binding did not systematically correlate with gene activation. Here, we explored the mechanism through which GCN5 controls transcription. First, we fine-mapped its GCN5 binding sites genome-wide and then used several global methodologies (ATAC-seq, ChIP-seq and RNA-seq) to assess the effect of GCN5 loss-of-function on the expression and epigenetic regulation of its target genes. These analyses provided evidence that GCN5 has a dual role in the regulation of H3K14ac levels in their 5' and 3' ends of its target genes. While the gcn5 mutation led to a genome-wide decrease of H3K14ac in the 5' end of the GCN5 down-regulated targets, it also led to an increase of H3K14ac in the 3' ends of GCN5 up-regulated targets. Furthermore, genome-wide changes in H3K14ac levels in the gcn5 mutant correlated with changes in H3K9ac at both 5' and 3' ends, providing evidence for a molecular link between the depositions of these two histone modifications. To understand the biological relevance of these regulations, we showed that GCN5 participates in the responses to biotic stress by repressing salicylic acid (SA) accumulation and SA-mediated immunity, highlighting the role of this protein in the regulation of the crosstalk between diverse developmental and stress-responsive physiological programs. Hence, our results demonstrate that GCN5, through the modulation of H3K14ac levels on its targets, controls the balance between biotic and abiotic stress responses and is a master regulator of plant-environmental interactions.Citation
GCN5 modulates salicylic acid homeostasis by regulating H3K14ac levels at the 5' and 3' ends of its target genes. (2020). Nucleic Acids Research. doi:10.1093/nar/gkaa369Sponsors
Agence Nationale de la Recherche (ANR) [EPICLISPE ANR-18-CE20-0015]; Institut Universitaire de France (IUF); Biotechnology and Biological Science Research Council [BB/L019345/1, BB/M017982/1 to V.N., in part]; V.N. is also supported by the Royal Society. Funding for open access charge: ANR research grant.Publisher
Oxford University Press (OUP)Journal
Nucleic acids researchPubMed ID
32396165PubMed Central ID
PMC7293002Additional Links
https://academic.oup.com/nar/article/48/11/5953/5836186ae974a485f413a2113503eed53cd6c53
10.1093/nar/gkaa369
Scopus Count
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