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dc.contributor.authorChodasiewicz, Monika
dc.contributor.authorSokolowska, Ewelina Maria
dc.contributor.authorNelson-Dittrich, Anna C.
dc.contributor.authorMasiuk, Aleksandra
dc.contributor.authorBeltran, Juan Camilo Moreno
dc.contributor.authorNelson, Andrew D.L.
dc.contributor.authorSkirycz, Aleksandra
dc.date.accessioned2020-11-24T05:30:15Z
dc.date.available2020-11-24T05:30:15Z
dc.date.issued2020-10-30
dc.date.submitted2020-08-17
dc.identifier.citationChodasiewicz, M., Sokolowska, E. M., Nelson-Dittrich, A. C., Masiuk, A., Beltran, J. C. M., Nelson, A. D. L., & Skirycz, A. (2020). Identification and Characterization of the Heat-Induced Plastidial Stress Granules Reveal New Insight Into Arabidopsis Stress Response. Frontiers in Plant Science, 11. doi:10.3389/fpls.2020.595792
dc.identifier.issn1664-462X
dc.identifier.doi10.3389/fpls.2020.595792
dc.identifier.urihttp://hdl.handle.net/10754/666088
dc.description.abstractPlants exhibit different physiological and molecular responses to adverse changes in their environment. One such molecular response is the sequestration of proteins, RNAs, and metabolites into cytoplasmic bodies called stress granules (cSGs). Here we report that, in addition to cSGs, heat stress also induces the formation of SG-like foci (cGs) in the chloroplasts of the model plant Arabidopsis thaliana. Similarly to the cSGs, (i) cpSG assemble rapidly in response to stress and disappear when the stress ceases, (ii) cpSG formation is inhibited by treatment with a translation inhibitor (lincomycin), and (iii) cpSG are composed of a stable core and a fluid outer shell. A previously published protocol for cSG extraction was successfully adapted to isolate cpSG, followed by protein, metabolite, and RNA analysis. Analogously to the cSGs, cpSG sequester proteins essential for SG formation, dynamics, and function, also including RNA-binding proteins with prion-like domain, ATPases and chaperones, and the amino acids proline and glutamic acid. However, the most intriguing observation relates to the cpSG localization of proteins, such as a complete magnesium chelatase complex, which is involved in photosynthetic acclimation to stress. These data suggest that cpSG have a role in plant stress tolerance.
dc.description.sponsorshipWe would like to acknowledge Prof. Alisdair Fernie for critical discussion of our work, Änne Michaelis for technical support (metabolite measurement), and Stella Kössler for assistance with the affinity experiment. Funding. The funding for the research included in the manuscript was fully provided by Max Planck Institute of Molecular Plant Physiology.
dc.publisherFrontiers Media SA
dc.relation.urlhttps://www.frontiersin.org/articles/10.3389/fpls.2020.595792/full
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleIdentification and Characterization of the Heat-Induced Plastidial Stress Granules Reveal New Insight Into Arabidopsis Stress Response
dc.typeArticle
dc.contributor.departmentCenter for Desert Agriculture, Biological and Environmental Science and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia
dc.identifier.journalFrontiers in Plant Science
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionMax Planck Institute of Molecular Plant Physiology, Golm, Germany
dc.contributor.institutionBoyce Thompson Institute, Cornell University, Ithaca, NY, United States
dc.identifier.volume11
kaust.personChodasiewicz, Monika
dc.date.accepted2020-10-07
dc.identifier.eid2-s2.0-85096223384
refterms.dateFOA2020-11-24T05:33:46Z


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This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution
or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.