Millimeter-Deep Detection of Single Shortwave-Infrared-Emitting Polymer Dots through Turbid Media
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
KAUST Grant NumberOSR-2016-2967-CRG5
Embargo End Date2021-11-18
Permanent link to this recordhttp://hdl.handle.net/10754/666078
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AbstractFluorescence imaging at longer wavelengths, especially in the shortwave-infrared (SWIR: 1000-1700 nm) region, leads to a substantial decrease in light attenuation, scattering, and background autofluorescence, thereby enabling enhanced penetration into biological tissues. The limited selection of fluorescent probes is a major bottleneck in SWIR fluorescence imaging. Here, we develop SWIR-emitting nanoparticles composed of donor-acceptor-type conjugated polymers. The bright SWIR fluorescence of the polymer dots (primarily attributable to their large absorption cross-section and high fluorescence saturation intensity (as high as 113 kW·cm-2)) enables the unprecedented detection of single particles as small as 14 nm through millimeter-thick turbid media. Unlike most SWIR-emitting nanomaterials, which have an excited-state lifetime in the range of microseconds to milliseconds, our polymer dots exhibit a subnanosecond excited-state lifetime. These characteristics enable us to demonstrate new time-gated single-particle imaging with a high signal-to-background ratio. These findings expand the range of potential applications of single-particle deep-tissue imaging.
CitationPiwoński, H., Wang, Y., Li, W., Michinobu, T., & Habuchi, S. (2020). Millimeter-Deep Detection of Single Shortwave-Infrared-Emitting Polymer Dots through Turbid Media. Nano Letters. doi:10.1021/acs.nanolett.0c03675
SponsorsThis study was supported by King Abdullah University of Science and Technology (KAUST) and the KAUST Office of Sponsored Research (OSR) under Award No. OSR-2016-2967-CRG5.
PublisherAmerican Chemical Society (ACS)