Millimeter-Deep Detection of Single Shortwave-Infrared-Emitting Polymer Dots through Turbid Media
Type
ArticleKAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
KAUST Grant Number
OSR-2016-2967-CRG5Date
2020-11-18Embargo End Date
2021-11-18Submitted Date
2020-09-10Permanent link to this record
http://hdl.handle.net/10754/666078
Metadata
Show full item recordAbstract
Fluorescence imaging at longer wavelengths, especially in the shortwave-infrared (SWIR: 1000-1700 nm) region, leads to a substantial decrease in light attenuation, scattering, and background autofluorescence, thereby enabling enhanced penetration into biological tissues. The limited selection of fluorescent probes is a major bottleneck in SWIR fluorescence imaging. Here, we develop SWIR-emitting nanoparticles composed of donor-acceptor-type conjugated polymers. The bright SWIR fluorescence of the polymer dots (primarily attributable to their large absorption cross-section and high fluorescence saturation intensity (as high as 113 kW·cm-2)) enables the unprecedented detection of single particles as small as 14 nm through millimeter-thick turbid media. Unlike most SWIR-emitting nanomaterials, which have an excited-state lifetime in the range of microseconds to milliseconds, our polymer dots exhibit a subnanosecond excited-state lifetime. These characteristics enable us to demonstrate new time-gated single-particle imaging with a high signal-to-background ratio. These findings expand the range of potential applications of single-particle deep-tissue imaging.Citation
Piwoński, H., Wang, Y., Li, W., Michinobu, T., & Habuchi, S. (2020). Millimeter-Deep Detection of Single Shortwave-Infrared-Emitting Polymer Dots through Turbid Media. Nano Letters. doi:10.1021/acs.nanolett.0c03675Sponsors
This study was supported by King Abdullah University of Science and Technology (KAUST) and the KAUST Office of Sponsored Research (OSR) under Award No. OSR-2016-2967-CRG5.Publisher
American Chemical Society (ACS)Journal
Nano LettersPubMed ID
33206524Additional Links
https://pubs.acs.org/doi/10.1021/acs.nanolett.0c03675ae974a485f413a2113503eed53cd6c53
10.1021/acs.nanolett.0c03675
Scopus Count
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