KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Online Publication Date2020-11-20
Print Publication Date2020-11-20
Embargo End Date2021-11-21
Permanent link to this recordhttp://hdl.handle.net/10754/666076
MetadataShow full item record
AbstractChIP-Seq is a widespread experimental method for determining the global enrichment of chromatin modifications and genome-associated factors. Whereas it is straightforward to compare the relative genomic distribution of these epigenetic features, researchers have also made efforts to compare their signal strength using external references for normalization. New work now suggests that these "spike-ins" could lead to inaccurate conclusions due to intrinsic issues of the methodology and instead calls for new criteria of experimental reporting that may permit internal standardization when certain parameters are fulfilled.
CitationFischle, W. (2020). A new approach for quantifying epigenetic landscapes. Journal of Biological Chemistry, 295(47), 15838–15839. doi:10.1074/jbc.h120.016430
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