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J. Biol. Chem.-2020-Fischle-15838-9.pdf
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ArticleAuthors
Fischle, Wolfgang
KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Date
2020-11-20Online Publication Date
2020-11-20Print Publication Date
2020-11-20Embargo End Date
2021-11-21Permanent link to this record
http://hdl.handle.net/10754/666076
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ChIP-Seq is a widespread experimental method for determining the global enrichment of chromatin modifications and genome-associated factors. Whereas it is straightforward to compare the relative genomic distribution of these epigenetic features, researchers have also made efforts to compare their signal strength using external references for normalization. New work now suggests that these "spike-ins" could lead to inaccurate conclusions due to intrinsic issues of the methodology and instead calls for new criteria of experimental reporting that may permit internal standardization when certain parameters are fulfilled.Citation
Fischle, W. (2020). A new approach for quantifying epigenetic landscapes. Journal of Biological Chemistry, 295(47), 15838–15839. doi:10.1074/jbc.h120.016430Journal
Journal of Biological ChemistryPubMed ID
33219165Additional Links
http://www.jbc.org/lookup/doi/10.1074/jbc.H120.016430ae974a485f413a2113503eed53cd6c53
10.1074/jbc.h120.016430
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