T cell receptor repertoire as a potential diagnostic marker for celiac disease.

Embargo End Date
2021-11-16

Type
Article

Authors
Yao, Ying
Zia, Asima
Neumann, Ralf Stefan
Pavlovic, Milena
Balaban, Gabriel
Lundin, Knut E A
Sandve, Geir Kjetil
Qiao, Shuo-Wang

KAUST Department
Biological and Environmental Sciences and Engineering (BESE) Division

Online Publication Date
2020-11-13

Print Publication Date
2021-01

Date
2020-11-13

Submitted Date
2020-06-04

Abstract
An individual's T cell repertoire is skewed towards some specificities as a result of past antigen exposure and subsequent clonal expansion. Identifying T cell receptor signatures associated with a disease is challenging due to the overall complexity of antigens and polymorphic HLA allotypes. In celiac disease, the antigen epitopes are well characterised and the specific HLA-DQ2-restricted T-cell repertoire associated with the disease has been explored in depth. By investigating T cell receptor repertoires of unsorted lamina propria T cells from 15 individuals, we provide the first proof-of-concept study showing that it could be possible to infer disease state by matching against a priori known disease-associated T cell receptor sequences.

Citation
Yao, Y., Zia, A., Neumann, R. S., Pavlovic, M., Balaban, G., Lundin, K. E. A., … Qiao, S.-W. (2020). T cell receptor repertoire as a potential diagnostic marker for celiac disease. Clinical Immunology, 108621. doi:10.1016/j.clim.2020.108621

Acknowledgements
The authors would like to thank Shiva Dahal-Koirala, Louise F Risnes and Ludvig M Sollid for access to the reference TCR database; and Victor Greiff for helpful comments and suggestions that improved the data processing.
This work is funded by Research Council of Norway (project 179573/V40 through the Centre of Excellence funding scheme and project 233885), and grants from the Stiftelsen Kristian Gerhard Jebsen (SKGJ-MED-017).

Publisher
Elsevier BV

Journal
Clinical Immunology

DOI
10.1016/j.clim.2020.108621

PubMed ID
33197618

Additional Links
https://linkinghub.elsevier.com/retrieve/pii/S1521661620307816

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