A nanobody-functionalized organic electrochemical transistor for the rapid detection of SARS-CoV-2 or MERS antigens at the physical limit
Alqahtani, Ahmed A.
Nazir Ahmad, Adeel
Alhamlan, Fatimah Saeed
Arold, Stefan T.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Chemical Science Program
Computational Bioscience Research Center (CBRC)
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Environmental Science and Engineering Program
KAUST Health, Thuwal 23955-6900, Saudi Arabia.
KAUST Solar Center (KSC)
King Abdullah University of Science and Technology (KAUST), Biological and Environmental Science and Engineering (BESE), Thuwal, 23955-6900, Saudi Arabia.
Physical Science and Engineering (PSE) Division
Structural Biology and Engineering
Permanent link to this recordhttp://hdl.handle.net/10754/666002
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AbstractThe COVID-19 pandemic highlights the need for rapid protein detection and quantification at the single-molecule level in a format that is simple and robust enough for widespread point-of-care applications. We here introduce a modular nanobody-organic electrochemical transistor architecture that enables the fast and specific detection and quantification of single-molecule to nanomolar protein antigen concentrations in complex bodily fluids. The sensor combines a new solution-processable organic semiconductor material in the transistor channel with the high-density and orientation-controlled bioconjugation of nanobody fusion proteins on disposable gate electrodes. It provides results after a 10 minutes exposure to 5 μL of unprocessed samples, maintains high specificity and single-molecule sensitivity in human saliva or serum, and is rapidly reprogrammed towards any protein target for which nanobodies exist. We demonstrate the use of this highly modular platform for the detection of green fluorescent protein, SARS-CoV-1/2, and MERS-CoV spike proteins and validate the sensor for COVID-19 screening in unprocessed clinical nasopharyngeal swab and saliva samples.
CitationGuo, K., Wustoni, S., Koklu, A., Díaz-Galicia, E., Moser, M., Hama, A., … Inal, S. (2020). A nanobody-functionalized organic electrochemical transistor for the rapid detection of SARS-CoV-2 or MERS antigens at the physical limit. doi:10.1101/2020.11.12.20228874
SponsorsThe research reported in this publication was supported by funding from King Abdullah University of Science and Technology (KAUST), Office of Sponsored Research (OSR), under award numbers REI/1/4204-01, REI/1/4229-01, OSR-2018-CRG7-3709, OSR-2018-CARF/CCF-3079, OSR-2015-CRG4-2572 and OSR-4106 CPF2019. We acknowledge EC FP7 Project SC2 (610115), EC H2020 (643791), and EPSRC Projects EP/G037515/1, EP/M005143/1, and EP/L016702/1. No payments or services from third parties were received.
PublisherCold Spring Harbor Laboratory