Show simple item record

dc.contributor.authorRchiad, ‍Zineb
dc.contributor.authorHaidar, Malak
dc.contributor.authorAnsari, Hifzur Rahman
dc.contributor.authorTajeri, Shahin
dc.contributor.authorMfarrej, Sara
dc.contributor.authorBen Rached, Fathia
dc.contributor.authorKaushik, Abhinav
dc.contributor.authorLangsley, Gordon
dc.contributor.authorPain, Arnab
dc.date.accessioned2020-11-09T09:21:15Z
dc.date.available2020-11-09T09:21:15Z
dc.date.issued2020-11-05
dc.date.submitted2020-06-09
dc.identifier.citationRchiad, Z., Haidar, M., Ansari, H. R., Tajeri, S., Mfarrej, S., Ben Rached, F., … Pain, A. (2020). Cover Image: Novel tumour suppressor roles for GZMA and RASGRP1 in Theileria annulata-transformed macrophages and human B lymphoma cells (Cellular Microbiology 12/2020). Cellular Microbiology, 22(12). doi:10.1111/cmi.13285
dc.identifier.issn1462-5814
dc.identifier.issn1462-5822
dc.identifier.doi10.1111/cmi.13285
dc.identifier.urihttp://hdl.handle.net/10754/665867
dc.description.abstractTheileria annulata is a tick-transmitted apicomplexan parasite that infects and transforms bovine leukocytes into disseminating tumours that cause a disease called tropical theileriosis. Using comparative transcriptomics we identified genes transcriptionally perturbed during Theileria-induced leukocyte transformation. Dataset comparisons highlighted a small set of genes associated with Theileria-transformed leukocyte dissemination. The roles of Granzyme A (GZMA) and RAS guanyl-releasing protein 1 (RASGRP1) were verified by CRISPR/Cas9-mediated knockdown. Knocking down expression of GZMA and RASGRP1 in attenuated macrophages led to a regain in their dissemination in Rag2/γC mice confirming their role as dissemination suppressors in vivo. We further evaluated the roles of GZMA and RASGRP1 in human B lymphomas by comparing the transcriptome of 934 human cancer cell lines to that of Theileria-transformed bovine host cells. We confirmed dampened dissemination potential of human B lymphomas that overexpress GZMA and RASGRP1. Our results provide evidence that GZMA and RASGRP1 have a novel tumour suppressor function in both T. annulata-infected bovine host leukocytes and in human B lymphomas.
dc.description.sponsorshipThis study was supported by a Competitive Research Grant from the Office for Sponsored Research (OSR-2015-CRG4-2610) at King Abdullah University of Science and Technology (KAUST) awarded to A. P. and G. L. Z. R. acknowledges KAUST for awarding her PhD studentship. S. T. was supported by a post-doctoral fellowship from ParaFrap (ANR-11-LABX-0024) and in addition to ANR-11-LABX-0024, G. L. also acknowledges core support from INSERM and the CNRS. We thank members of the Bioscience Core Laboratory (BCL) at KAUST for producing the raw sequencing datasets. We also thank Franck Letourneur of the genomics platform at the Cochin Institute (GENOM'IC) for quantifying the pJET-ama-1 plasmid.
dc.publisherWiley
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/10.1111/cmi.13285
dc.rightsThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
dc.rights.uriCreative Commons Attribution-NonCommercial
dc.titleCover Image: Novel tumour suppressor roles for GZMA and RASGRP1 in Theileria annulata-transformed macrophages and human B lymphoma cells (Cellular Microbiology 12/2020)
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.contributor.departmentPathogen Genomics Laboratory
dc.identifier.journalCellular Microbiology
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionLaboratoire de Biologie Comparative des Apicomplexes, Facult é de Médecine, Université Paris Descartes – Sorbonne Paris Cité, Paris, France.
dc.contributor.institutionINSERM U1016, CNRS UMR8104, Cochin Institute, Paris, France.
dc.contributor.institutionCentre de Coalition, Innovation, et de prévention des Epidémies au Maroc (CIPEM), Mohammed VI Polytechnic University (UM6P), Ben Guerir, Morocco.
dc.contributor.institutionKing Abdullah International Medical Research Center (KAIMRC), King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Jeddah, Saudi Arabia.
dc.contributor.institutionGlobal Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo, Japan.
dc.identifier.volume22
dc.identifier.issue12
kaust.personRchiad, ‍Zineb
kaust.personHaidar, Malak
kaust.personAnsari, Hifzur Rahman
kaust.personMfarrej, Sara
kaust.personBen Rached, Fathia
kaust.personKaushik, Abhinav
kaust.personPain, Arnab
kaust.grant.numberOSR-2015-CRG4-2610
dc.date.accepted2020-06-09
refterms.dateFOA2020-11-09T09:21:45Z
kaust.acknowledged.supportUnitBioscience Core Laboratory (BCL)
kaust.acknowledged.supportUnitCompetitive Research
kaust.acknowledged.supportUnitOSR
kaust.acknowledged.supportUnitSponsored Research
dc.date.published-online2020-11-05
dc.date.published-print2020-12


Files in this item

Thumbnail
Name:
cmi.13255 (1).pdf
Size:
2.459Mb
Format:
PDF
Description:
Published version

This item appears in the following Collection(s)

Show simple item record