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dc.contributor.authorPascelli, Cecília
dc.contributor.authorLaffy, Patrick W
dc.contributor.authorBotté, Emmanuelle
dc.contributor.authorKupresanin, Marija
dc.contributor.authorRattei, Thomas
dc.contributor.authorLurgi, Miguel
dc.contributor.authorRavasi, Timothy
dc.contributor.authorWebster, Nicole S.
dc.date.accessioned2020-11-02T12:30:16Z
dc.date.available2020-11-02T12:30:16Z
dc.date.issued2020-10-02
dc.date.submitted2020-07-08
dc.identifier.citationPascelli, C., Laffy, P. W., Botté, E., Kupresanin, M., Rattei, T., Lurgi, M., … Webster, N. S. (2020). Viral ecogenomics across the Porifera. Microbiome, 8(1). doi:10.1186/s40168-020-00919-5
dc.identifier.issn2049-2618
dc.identifier.pmid33008461
dc.identifier.doi10.1186/s40168-020-00919-5
dc.identifier.urihttp://hdl.handle.net/10754/665761
dc.description.abstractBACKGROUND:Viruses directly affect the most important biological processes in the ocean via their regulation of prokaryotic and eukaryotic populations. Marine sponges form stable symbiotic partnerships with a wide diversity of microorganisms and this high symbiont complexity makes them an ideal model for studying viral ecology. Here, we used morphological and molecular approaches to illuminate the diversity and function of viruses inhabiting nine sponge species from the Great Barrier Reef and seven from the Red Sea. RESULTS:Viromic sequencing revealed host-specific and site-specific patterns in the viral assemblages, with all sponge species dominated by the bacteriophage order Caudovirales but also containing variable representation from the nucleocytoplasmic large DNA virus families Mimiviridae, Marseilleviridae, Phycodnaviridae, Ascoviridae, Iridoviridae, Asfarviridae and Poxviridae. Whilst core viral functions related to replication, infection and structure were largely consistent across the sponge viromes, functional profiles varied significantly between species and sites largely due to differential representation of putative auxiliary metabolic genes (AMGs) and accessory genes, including those associated with herbicide resistance, heavy metal resistance and nylon degradation. Furthermore, putative AMGs varied with the composition and abundance of the sponge-associated microbiome. For instance, genes associated with antimicrobial activity were enriched in low microbial abundance sponges, genes associated with nitrogen metabolism were enriched in high microbial abundance sponges and genes related to cellulose biosynthesis were enriched in species that host photosynthetic symbionts. CONCLUSIONS:Our results highlight the diverse functional roles that viruses can play in marine sponges and are consistent with our current understanding of sponge ecology. Differential representation of putative viral AMGs and accessory genes across sponge species illustrate the diverse suite of beneficial roles viruses can play in the functional ecology of these complex reef holobionts. Video Abstract.
dc.description.sponsorshipWe thank Dr. Rachid Sougrat and Dr. Ptissam Bergam for support with the operation of the TEM and Dr. Muhammad Azmi Abdul Wahab and Dr. Holly Bennett for sample collection support. The authors acknowledge the Bindal and Wulgurukaba Peoples of Davies Reef, as the Traditional Owners of the sea country where this work took place. We pay our respects to their elders past, present and emerging and we acknowledge their continuing spiritual connection to their sea country.
dc.publisherSpringer Nature
dc.relation.urlhttps://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-020-00919-5
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleViral ecogenomics across the Porifera.
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.contributor.departmentInnovation and Economic Development
dc.contributor.departmentIntegrative Systems Biology Lab
dc.identifier.journalMicrobiome
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionAustralian Institute of Marine Science PMB No.3, Townsville MC Townsville, Queensland 4810 Australia
dc.contributor.institutionAIMS@JCU Townsville, Queensland Australia
dc.contributor.institutionJames Cook University Townsville Australia
dc.contributor.institutionDepartment of Microbiology and Ecosystem Science, Division of Computational Systems Biology, University of Vienna Vienna Austria
dc.contributor.institutionBiosciences Department, University of Swansea Swansea Wales
dc.contributor.institutionAustralian Centre for Ecogenomics, University of Queensland Brisbane Australia
dc.identifier.volume8
dc.identifier.issue1
kaust.personKupresanin, Marija
kaust.personRavasi, Timothy
dc.date.accepted2020-09-08
dc.relation.issupplementedbyDOI:10.6084/m9.figshare.c.5141427
dc.identifier.eid2-s2.0-85092300773
refterms.dateFOA2020-11-02T12:31:29Z
display.relations<b>Is Supplemented By:</b><br/> <ul><li><i>[Dataset]</i> <br/> Pascelli, C., Laffy, P. W., Botté, E., Kupresanin, M., Rattei, T., Lurgi, M., Ravasi, T., &amp; Webster, N. S. (2020). <i>Viral ecogenomics across the Porifera</i>. figshare. https://doi.org/10.6084/M9.FIGSHARE.C.5141427. DOI: <a href="https://doi.org/10.6084/m9.figshare.c.5141427" >10.6084/m9.figshare.c.5141427</a> Handle: <a href="http://hdl.handle.net/10754/667214" >10754/667214</a></a></li></ul>
dc.date.published-online2020-10-02
dc.date.published-print2020-12


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This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
Except where otherwise noted, this item's license is described as This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.