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    Fluorescence-assisted real-time study of magnetically immobilized enzyme stability in a crossflow membrane bioreactor

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    5684- Xflo fluorescence-sn.pdf
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    Type
    Article
    Authors
    Gebreyohannes, Abaynesh Yihdego cc
    Geens, T.
    Kubarev, A. cc
    Roeffaers, M.
    Naessens, W.
    Swusten, T.
    Verbiest, T.
    Nopens, I. cc
    Nunes, Suzana Pereira cc
    Vankelecom, I. F.J.
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Environmental Science and Engineering Program
    Nanostructured Polymeric Membrane Lab
    Date
    2020-10-04
    Online Publication Date
    2020-10-04
    Print Publication Date
    2020-10
    Embargo End Date
    2021-10-04
    Submitted Date
    2020-07-07
    Permanent link to this record
    http://hdl.handle.net/10754/665684
    
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    Abstract
    The detailed structures and distribution of enzymatically active magnetic-responsive dynamic layers (EnzSP) were investigated for the first time in a crossflow superparamagnetic biocatalytic membrane reactor (XF-BMRSP). The trade-off between a higher mass transfer rate, lower fouling tendency, and preventing washout of the dynamic layer, highly depends on the balance of the various forces that act on the EnzSP. The real-time visual inspection of the biointerface was realized through the design and fabrication of an adapted crossflow system, guided by computational fluid dynamics (CFD) simulations. Time-resolved images of the dynamic layer under a broad range of operational conditions was obtained using fluorescence microscopy. The deposition, dispersion and stability of the dynamic layer was mainly governed by the external magnetic force. The shear force did not cause significant particle washout when a buffer solution was recirculated without permeation even under a turbulent flow regime (6.4 cm/s ∼ Re = 5200). The removal of the external magnetic force after initial magnetic immobilization of the EnzSP, or the substitution of a smooth flow velocity by the propagation of an impulse flow, significantly affected the stability of the dynamic layer. Although membrane fouling occurs at the membrane-solution interface, where a laminar flow regime prevails, most membrane fouling models are based on turbulent flow. Therefore, the detailed, time-resolved images obtained here can provide solid foundation for the development of theoretical models that can describe the membrane fouling under the more representative laminar flow regime.
    Citation
    Gebreyohannes, A. Y., Geens, T., Kubarev, A., Roeffaers, M., Naessens, W., Swusten, T., … Vankelecom, I. F. J. (2020). Fluorescence-assisted real-time study of magnetically immobilized enzyme stability in a crossflow membrane bioreactor. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 125687. doi:10.1016/j.colsurfa.2020.125687
    Sponsors
    We are grateful to the Belgian Federal Government for an IAP grant (FS2) and KU Leuven for an IOF-KP grant (13/004)
    Publisher
    Elsevier BV
    Journal
    Colloids and Surfaces A: Physicochemical and Engineering Aspects
    DOI
    10.1016/j.colsurfa.2020.125687
    Additional Links
    https://linkinghub.elsevier.com/retrieve/pii/S0927775720312802
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.colsurfa.2020.125687
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Environmental Science and Engineering Program

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