Fluorescence-assisted real-time study of magnetically immobilized enzyme stability in a crossflow membrane bioreactor
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Gebreyohannes, Abaynesh Yihdego
Geens, T.
Kubarev, A.

Roeffaers, M.
Naessens, W.
Swusten, T.
Verbiest, T.
Nopens, I.

Nunes, Suzana Pereira

Vankelecom, I. F.J.
KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionEnvironmental Science and Engineering Program
Nanostructured Polymeric Membrane Lab
Date
2020-10-04Online Publication Date
2020-10-04Print Publication Date
2020-10Embargo End Date
2021-10-04Submitted Date
2020-07-07Permanent link to this record
http://hdl.handle.net/10754/665684
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The detailed structures and distribution of enzymatically active magnetic-responsive dynamic layers (EnzSP) were investigated for the first time in a crossflow superparamagnetic biocatalytic membrane reactor (XF-BMRSP). The trade-off between a higher mass transfer rate, lower fouling tendency, and preventing washout of the dynamic layer, highly depends on the balance of the various forces that act on the EnzSP. The real-time visual inspection of the biointerface was realized through the design and fabrication of an adapted crossflow system, guided by computational fluid dynamics (CFD) simulations. Time-resolved images of the dynamic layer under a broad range of operational conditions was obtained using fluorescence microscopy. The deposition, dispersion and stability of the dynamic layer was mainly governed by the external magnetic force. The shear force did not cause significant particle washout when a buffer solution was recirculated without permeation even under a turbulent flow regime (6.4 cm/s ∼ Re = 5200). The removal of the external magnetic force after initial magnetic immobilization of the EnzSP, or the substitution of a smooth flow velocity by the propagation of an impulse flow, significantly affected the stability of the dynamic layer. Although membrane fouling occurs at the membrane-solution interface, where a laminar flow regime prevails, most membrane fouling models are based on turbulent flow. Therefore, the detailed, time-resolved images obtained here can provide solid foundation for the development of theoretical models that can describe the membrane fouling under the more representative laminar flow regime.Citation
Gebreyohannes, A. Y., Geens, T., Kubarev, A., Roeffaers, M., Naessens, W., Swusten, T., … Vankelecom, I. F. J. (2020). Fluorescence-assisted real-time study of magnetically immobilized enzyme stability in a crossflow membrane bioreactor. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 125687. doi:10.1016/j.colsurfa.2020.125687Sponsors
We are grateful to the Belgian Federal Government for an IAP grant (FS2) and KU Leuven for an IOF-KP grant (13/004)Publisher
Elsevier BVAdditional Links
https://linkinghub.elsevier.com/retrieve/pii/S0927775720312802ae974a485f413a2113503eed53cd6c53
10.1016/j.colsurfa.2020.125687