Enrichment of salt-tolerant CO2-fixing communities in microbial electrosynthesis systems using porous ceramic hollow tube wrapped with carbon cloth as cathode and for CO2 supply.
Type
ArticleAuthors
AlQahtani, Manal Faisal
Bajracharya, Suman

Katuri, Krishna
Ali, Muhammad
Xu, Jiajie
Alarawi, Mohammed S
Saikaly, Pascal

KAUST Department
Biological and Environmental Science and Engineering (BESE) Division, Water Desalination and Reuse Center, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.Biological and Environmental Sciences and Engineering (BESE) Division
Computational Biosciences Research Center, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.
Environmental Biotechnology Research Group
Environmental Science and Engineering Program
Water Desalination and Reuse Research Center (WDRC)
KAUST Grant Number
URF/1/2985-01-01Date
2020-10-05Online Publication Date
2020-10-05Print Publication Date
2020-10Embargo End Date
2022-10-20Submitted Date
2020-06-25Permanent link to this record
http://hdl.handle.net/10754/665650
Metadata
Show full item recordAbstract
Microbial inocula from marine origins are less explored for CO2 reduction in microbial electrosynthesis (MES) system, although effective CO2-fixing communities in marine environments are well-documented. We explored natural saline habitats, mainly salt marsh (SM) and mangrove (M) sediments, as potential inoculum sources for enriching salt-tolerant CO2 reducing community using two enrichment strategies: H2:CO2 (80:20) enrichment in serum vials and enrichment in cathode chamber of MES reactors operated at -1.0 V vs. Ag/AgCl. Porous ceramic hollow tube wrapped with carbon cloth was used as cathode and for direct CO2 delivery to CO2 reducing communities growing on the cathode surface. Methanogenesis was dominant in both the M- and SM-seeded MES and the methanogenic Archaea Methanococcus was the most dominant genus. Methane production was slightly higher in the SM-seeded MES (4.9 ± 1.7 mmol) compared to the M-seeded MES (3.8 ± 1.1 mmol). In contrast, acetate production was almost two times higher in the M-seeded MES (3.1 ± 0.9 mmol) than SM-seeded MES (1.5 ± 1.3 mmol). The high relative abundance of the genus Acetobacterium in the M-seeded serum vials correlates with the high acetate production obtained. The different enrichment strategies affected the community composition, though the communities in MES reactors and serum vials were performing similar functions (methanogenesis and acetogenesis). Despite similar operating conditions, the microbial community composition of M-seeded serum vials and MES reactors differed from the SM-seeded serum vials and MES reactors, supporting the importance of inoculum source in the evolution of CO2-reducing microbial communities.Citation
Alqahtani, M. F., Bajracharya, S., Katuri, K. P., Ali, M., Xu, J., Alarawi, M. S., & Saikaly, P. E. (2020). Enrichment of salt-tolerant CO2–fixing communities in microbial electrosynthesis systems using porous ceramic hollow tube wrapped with carbon cloth as cathode and for CO2 supply. Science of The Total Environment, 142668. doi:10.1016/j.scitotenv.2020.142668Sponsors
This work was supported by Competitive Research Grant (URF/1/2985-01-01) from King Abdullah University of Science and Technology to P.E.S.Publisher
Elsevier BVJournal
Science of The Total EnvironmentPubMed ID
33077225Additional Links
https://linkinghub.elsevier.com/retrieve/pii/S0048969720361970ae974a485f413a2113503eed53cd6c53
10.1016/j.scitotenv.2020.142668
Scopus Count
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