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dc.contributor.authorBarylyuk, Konstantin
dc.contributor.authorKoreny, Ludek
dc.contributor.authorKe, Huiling
dc.contributor.authorButterworth, Simon
dc.contributor.authorCrook, Oliver M
dc.contributor.authorLassadi, Imen
dc.contributor.authorGupta, Vipul
dc.contributor.authorTromer, Eelco
dc.contributor.authorMourier, Tobias
dc.contributor.authorStevens, Tim J
dc.contributor.authorBreckels, Lisa M
dc.contributor.authorPain, Arnab
dc.contributor.authorLilley, Kathryn S
dc.contributor.authorWaller, Ross F
dc.identifier.citationBarylyuk, K., Koreny, L., Ke, H., Butterworth, S., Crook, O. M., Lassadi, I., … Waller, R. F. (2020). A Comprehensive Subcellular Atlas of the Toxoplasma Proteome via hyperLOPIT Provides Spatial Context for Protein Functions. Cell Host & Microbe. doi:10.1016/j.chom.2020.09.011
dc.description.abstractApicomplexan parasites cause major human disease and food insecurity. They owe their considerable success to highly specialized cell compartments and structures. These adaptations drive their recognition, nondestructive penetration, and elaborate reengineering of the host's cells to promote their growth, dissemination, and the countering of host defenses. The evolution of unique apicomplexan cellular compartments is concomitant with vast proteomic novelty. Consequently, half of apicomplexan proteins are unique and uncharacterized. Here, we determine the steady-state subcellular location of thousands of proteins simultaneously within the globally prevalent apicomplexan parasite Toxoplasma gondii. This provides unprecedented comprehensive molecular definition of these unicellular eukaryotes and their specialized compartments, and these data reveal the spatial organizations of protein expression and function, adaptation to hosts, and the underlying evolutionary trajectories of these pathogens.
dc.description.sponsorshipThis work was supported by the Medical Research Council, United Kingdom, MR/M011690/1 to R.F.W.; King Abdullah University of Science and Technology (KAUST), Saudi Arabia, OSR-2015-CRG4-2610 to A.P., R.F.W., and K.S.L.; Wellcome Trust, United Kingdom, Investigator award 214298/Z/18/Z to R.F.W.; an Isaac Newton Trust-Leverhulme, Early Career Fellowship ECF-2015-562 to K.B; and KAUST faculty baseline funding (BAS/1/1020-01-01) to A.P. Mass spectrometry data were acquired by Mike Deery at the Cambridge Centre of Proteomics, and we thank Laurent Gatto for useful discussions.
dc.publisherElsevier BV
dc.rightsThis is an open access article under the CC BY license.
dc.titleA Comprehensive Subcellular Atlas of the Toxoplasma Proteome via hyperLOPIT Provides Spatial Context for Protein Functions.
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentPathogen Genomics Laboratory
dc.identifier.journalCell host & microbe
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDepartment of Biochemistry, University of Cambridge, Cambridge CB2 1QW, UK.
dc.contributor.institutionMilner Therapeutics Institute, Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge CB20 0AW, UK.
dc.contributor.institutionMRC Biostatistics Unit, Cambridge Institute for Public Health, Cambridge CB2 0SR, UK.
dc.contributor.institutionMRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK.
dc.contributor.institutionGlobal Station for Zoonosis Control, Gi-CoRE, Hokkaido University, Sapporo 060-0808, Japan.
dc.contributor.institutionNuffield Division of Clinical Laboratory Sciences (NDCLS), University of Oxford, Oxford OX3 9DU, UK.
kaust.personMourier, Tobias
kaust.personPain, Arnab

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