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dc.contributor.authorSumida, Noriyuki
dc.contributor.authorSifakis, Emmanouil G
dc.contributor.authorKiani, Narsis A
dc.contributor.authorRonnegren, Anna Lewandowska
dc.contributor.authorScholz, Barbara A
dc.contributor.authorVestlund, Johanna
dc.contributor.authorGomez-Cabrero, David
dc.contributor.authorTegner, Jesper
dc.contributor.authorGöndör, Anita
dc.contributor.authorOhlsson, Rolf
dc.date.accessioned2020-10-18T11:04:07Z
dc.date.available2020-10-18T11:04:07Z
dc.date.issued2020-10-13
dc.date.submitted2020-07-09
dc.identifier.citationSumida, N., Sifakis, E. G., Kiani, N. A., Ronnegren, A. L., Scholz, B. A., Vestlund, J., … Ohlsson, R. (2020). MYC as a driver of stochastic chromatin networks: implications for the fitness of cancer cells. Nucleic Acids Research. doi:10.1093/nar/gkaa817
dc.identifier.issn0305-1048
dc.identifier.issn1362-4962
dc.identifier.pmid33051686
dc.identifier.doi10.1093/nar/gkaa817
dc.identifier.urihttp://hdl.handle.net/10754/665615
dc.description.abstractAbstract The relationship between stochastic transcriptional bursts and dynamic 3D chromatin states is not well understood. Using an innovated, ultra-sensitive technique, we address here enigmatic features underlying the communications between MYC and its enhancers in relation to the transcriptional process. MYC thus interacts with its flanking enhancers in a mutually exclusive manner documenting that enhancer hubs impinging on MYC detected in large cell populations likely do not exist in single cells. Dynamic encounters with pathologically activated enhancers responsive to a range of environmental cues, involved <10% of active MYC alleles at any given time in colon cancer cells. Being the most central node of the chromatin network, MYC itself likely drives its communications with flanking enhancers, rather than vice versa. We submit that these features underlie an acquired ability of MYC to become dynamically activated in response to a diverse range of environmental cues encountered by the cell during the neoplastic process.
dc.description.sponsorshipThe authors would like to acknowledge initial assistance from Drs Alejandro Woodbridge and Peter J Svensson as well as support from Science for Life Laboratory, the National Genomics Infrastructure, NGI, and Uppmax for providing assistance in massive parallel sequencing and computational infrastructure as well as the extensive data sets from ENCODE.
dc.description.sponsorshipSwedish Research Council [VR 2017-04670 and VR 2016-03108]; Swedish Childhood Cancer Fund [PR2017-0132]; Swedish Cancer Society [CAN2017/515, CAN 2016/708]; Lundberg Foundation [2018-0138]; Karolinska Institutet; Novo Nordisk Foundation [NNF16OC0021512]; Cancer Society in Stockholm (2018–2021 and 2019–2021); KA Wallenberg Foundation [KAW 2017.0077]. Funding for open access charge: KA Wallenberg Foundation.
dc.publisherOxford University Press (OUP)
dc.relation.urlhttps://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa817/5922798
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.titleMYC as a driver of stochastic chromatin networks: implications for the fitness of cancer cells
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.identifier.journalNucleic Acids Research
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDepartment of Oncology-Pathology, Karolinska Institutet, Karolinska University Hospital, Z1:00, SE-171 76 Stockholm, Sweden
dc.contributor.institutionUnit of Computational Medicine, Department of Medicine, Center for Molecular Medicine, Karolinska Institutet, Karolinska University Hospital, L8:05, SE-171 76, Stockholm, Sweden
dc.contributor.institutionMucosal and Salivary Biology Division, King's College London Dental Institute, London SE1 9RT, UK
dc.contributor.institutionScience for Life Laboratory, Tomtebodavägen 23A, SE-17165, Solna, Sweden
kaust.personTegner, Jesper
dc.date.accepted2020-10-11
refterms.dateFOA2020-10-18T11:05:34Z
dc.date.published-online2020-10-13
dc.date.published-print2020-11-04


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This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.