Population-scale proteome variation in human induced pluripotent stem cells
AuthorsMirauta, Bogdan Andrei
Seaton, Daniel D.
Bonder, Marc Jan
Agu, Chukwuma A.
Gaffney, Daniel J.
Kirton, Christopher M.
McCarthy, Shane A.
Paolo Casale, Francesco
Harrison, Peter W.
Watt, Fiona M.
Ouwehand, Willem H.
Lamond, Angus I.
KAUST DepartmentProteomics and Protein Expression
Permanent link to this recordhttp://hdl.handle.net/10754/665584
MetadataShow full item record
AbstractHuman disease phenotypes are driven primarily by alterations in protein expression and/or function. To date, relatively little is known about the variability of the human proteome in populations and how this relates to variability in mRNA expression and to disease loci. Here, we present the first comprehensive proteomic analysis of human induced pluripotent stem cells (iPSC), a key cell type for disease modelling, analysing 202 iPSC lines derived from 151 donors, with integrated transcriptome and genomic sequence data from the same lines. We characterised the major genetic and non-genetic determinants of proteome variation across iPSC lines and assessed key regulatory mechanisms affecting variation in protein abundance. We identified 654 protein quantitative trait loci (pQTLs) in iPSCs, including disease-linked variants in protein-coding sequences and variants with trans regulatory effects. These include pQTL linked to GWAS variants that cannot be detected at the mRNA level, highlighting the utility of dissecting pQTL at peptide level resolution.
CitationMirauta, B. A., Seaton, D. D., Bensaddek, D., Brenes, A., Bonder, M. J., … Kilpinen, H. (2020). Population-scale proteome variation in human induced pluripotent stem cells. eLife, 9. doi:10.7554/elife.57390
PublishereLife Sciences Publications, Ltd
Except where otherwise noted, this item's license is described as This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.