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dc.contributor.authorBauer, Meike
dc.contributor.authorWunderlich, Lukas
dc.contributor.authorWeinzierl, Florian
dc.contributor.authorLei, Yongjiu
dc.contributor.authorDuerkop, Axel
dc.contributor.authorAlshareef, Husam N.
dc.contributor.authorBaeumner, Antje J.
dc.date.accessioned2020-10-04T09:02:19Z
dc.date.available2020-10-04T09:02:19Z
dc.date.issued2020-09-28
dc.date.submitted2020-06-29
dc.identifier.citationBauer, M., Wunderlich, L., Weinzierl, F., Lei, Y., Duerkop, A., Alshareef, H. N., & Baeumner, A. J. (2020). Electrochemical multi-analyte point-of-care perspiration sensors using on-chip three-dimensional graphene electrodes. Analytical and Bioanalytical Chemistry. doi:10.1007/s00216-020-02939-4
dc.identifier.issn1618-2650
dc.identifier.issn1618-2642
dc.identifier.doi10.1007/s00216-020-02939-4
dc.identifier.urihttp://hdl.handle.net/10754/665420
dc.description.abstractMulti-analyte sensing using exclusively laser-induced graphene (LIG)-based planar electrode systems was developed for sweat analysis. LIG provides 3D structures of graphene, can be manufactured easier than any other carbon electrode also on large scale, and in form of electrodes: hence, it is predestinated for affordable, wearable point-of-care sensors. Here, it is demonstrated that LIG facilitates all three electrochemical sensing strategies (voltammetry, potentiometry, impedance) in a multi-analyte system for sweat analysis. A potentiometric potassium-ion-selective electrode in combination with an electrodeposited Ag/AgCl reference electrode (RE) enabled the detection of potassium ions in the entire physiologically relevant range (1 to 500 mM) with a fast response time, unaffected by the presence of main interfering ions and sweat-collecting materials. A kidney-shaped interdigitated LIG electrode enabled the determination of the overall electrolyte concentration by electrochemical impedance spectroscopy at a fixed frequency. Enzyme-based strategies with amperometric detection share a common RE and were realized with Prussian blue as electron mediator and biocompatible chitosan for enzyme immobilization and protection of the electrode. Using glucose and lactate oxidases, lower limits of detection of 13.7 ± 0.5 μM for glucose and 28 ± 3 μM for lactate were obtained, respectively. The sensor showed a good performance at different pH, with sweat-collecting tissues, on a model skin system and furthermore in synthetic sweat as well as in artificial tear fluid. Response time for each analytical cycle totals 75 s, and hence allows a quasi-continuous and simultaneous monitoring of all analytes. This multi-analyte all-LIG system is therefore a practical, versatile, and most simple strategy for point-of-care applications and has the potential to outcompete standard screen-printed electrodes. [Figure not available: see fulltext.].
dc.description.sponsorshipWe acknowledge Marcel Simsek for recording the SEM images, Arne Behrent for the Raman measurements, and Vanessa Tomanek for drawing the graphical abstract.
dc.description.sponsorshipOpen Access funding provided by Projekt DEAL. The authors acknowledge partial funding and availability of the laser scriber through a grant by the King Abdullah University of Science and Technology (KAUST) Sensor Initiative (Grant # CRF-2015-SENSOR-2709).
dc.publisherSpringer Nature
dc.relation.urlhttp://link.springer.com/10.1007/s00216-020-02939-4
dc.rightsThis article is open access licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleElectrochemical multi-analyte point-of-care perspiration sensors using on-chip three-dimensional graphene electrodes
dc.typeArticle
dc.contributor.departmentFunctional Nanomaterials and Devices Research Group
dc.contributor.departmentMaterial Science and Engineering Program
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.identifier.journalAnalytical and Bioanalytical Chemistry
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionInstitute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040, Regensburg, Germany
dc.contributor.institutionDepartment of Biological and Environmental Engineering, Cornell University, Ithaca, NY, 14853, USA
kaust.personLei, Yongjiu
kaust.personAlshareef, Husam N.
kaust.grant.numberCRF-2015-SENSOR-2709
dc.date.accepted2020-09-03
dc.identifier.eid2-s2.0-85091612804
refterms.dateFOA2020-10-04T09:03:03Z


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This article is open access licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing,
adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
Except where otherwise noted, this item's license is described as This article is open access licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.