Striatal infusion of cholesterol promotes dose-dependent behavioral benefits and exerts disease-modifying effects in Huntington's disease mice
Di Paolo, Eleonora
Bocchi, Vittoria D
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Online Publication Date2020-09-22
Print Publication Date2020-10-07
Permanent link to this recordhttp://hdl.handle.net/10754/665339
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AbstractA variety of pathophysiological mechanisms are implicated in Huntington's disease (HD). Among them, reduced cholesterol biosynthesis has been detected in the HD mouse brain from pre-symptomatic stages, leading to diminished cholesterol synthesis, particularly in the striatum. In addition, systemic injection of cholesterol-loaded brain-permeable nanoparticles ameliorates synaptic and cognitive function in a transgenic mouse model of HD. To identify an appropriate treatment regimen and gain mechanistic insights into the beneficial activity of exogenous cholesterol in the HD brain, we employed osmotic mini-pumps to infuse three escalating doses of cholesterol directly into the striatum of HD mice in a continuous and rate-controlled manner. All tested doses prevented cognitive decline, while amelioration of disease-related motor defects was dose-dependent. In parallel, we found morphological and functional recovery of synaptic transmission involving both excitatory and inhibitory synapses of striatal medium spiny neurons. The treatment also enhanced endogenous cholesterol biosynthesis and clearance of mutant Huntingtin aggregates. These results indicate that cholesterol infusion to the striatum can exert a dose-dependent, disease-modifying effect and may be therapeutically relevant in HD.
CitationBirolini, G., Valenza, M., Di Paolo, E., Vezzoli, E., Talpo, F., Maniezzi, C., … Cattaneo, E. (2020). Striatal infusion of cholesterol promotes dose-dependent behavioral benefits and exerts disease-modifying effects in Huntington’s disease mice. EMBO Molecular Medicine. doi:10.15252/emmm.202012519
JournalEMBO Molecular Medicine
Except where otherwise noted, this item's license is described as This is an open access article under theterms of the Creative Commons Attribution 4.0License, which permits use, distribution and reproduc-tion in any medium, provided the original work isproperly cited.
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