Lipids and fatty acid composition in the crustacean model organism Artemia sp. as influenced by poly-β-hydroxybutyrate (PHB) supplementation

Abstract
The effects of dietary poly-β-hydroxybutyrate (PHB) on the lipid and fatty acids (FA) in crustaceans were investigated using Artemia as model species. Supplying PHB either in crystalline or amorphous form significantly increased the whole-body lipid contents of starved Artemia. Co-supplying dietary PHB with bacterial PHB degrader Comamonas testosteroni at 106 cells ml−1 for 24 hr also significantly increased this parameter. These findings have important implications on the lipid-saving effects of PHB in crustacean tissues. Dietary PHB, however, did not contribute to the increase in docosahexaenoic acid (DHA) of the naturally DHA-deprived Artemia. Alternative strategy of co-supplying dietary PHB with highly unsaturated fatty acid (HUFA)-rich emulsion for 24 hr effectively improved the nutritional contents of Artemia and at the same time assured efficient delivery of PHB to the larval predator. According to a 13C stable isotope tracer study, the significantly higher δ13C levels measured in Artemia 2 hr postfeeding with 13C-labelled R. eutropha DSM545 indicate that PHB offered in natural matrix is rapidly assimilated. Overall, this study demonstrated the lipid-saving effects of PHB in Artemia. PHB assimilation following gastrointestinal degradation could attribute to its reported beneficial effects in various aquatic farmed species.

Citation
Ludevese-Pascual, G., Leopoldo Laranja, J., Ahmed, F., Amar, E., De Troch, M., Bossier, P., & De Schryver, P. (2020). Lipids and fatty acid composition in the crustacean model organism Artemia sp. as influenced by poly-β-hydroxybutyrate (PHB) supplementation. Aquaculture Nutrition, 26(6), 2235–2244. doi:10.1111/anu.13160

Publisher
Wiley

Journal
Aquaculture Nutrition

DOI
10.1111/anu.13160

Additional Links
https://onlinelibrary.wiley.com/doi/10.1111/anu.13160

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