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    3D Printed Microneedle Array for Electroporation

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    Name:
    3D Printed Microneedle Array for Electroporation.pdf
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    3.666Mb
    Format:
    PDF
    Description:
    Accepted manuscript
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    Type
    Conference Paper
    Authors
    Moussi, Khalil cc
    Kavaldzhiev, Mincho cc
    Perez, Jose E. cc
    Alsharif, Nouf cc
    Merzaban, Jasmeen cc
    Kosel, Jürgen cc
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience Program
    Chemical Engineering Program
    Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
    Electrical Engineering Program
    Physical Science and Engineering (PSE) Division
    Sensing, Magnetism and Microsystems Lab
    Date
    2020-08-28
    Online Publication Date
    2020-08-28
    Print Publication Date
    2020-07
    Permanent link to this record
    http://hdl.handle.net/10754/665215
    
    Metadata
    Show full item record
    Abstract
    In-vitro transfection of cells by electroporation is a widely used approach in cell biology and medicine. The transfection method is highly dependent on the cell culture’s electrical resistance, which is strongly determined by differences in the membranes, but also on the morphology of the electrodes. Microneedle (MN)-based electrodes have been used to concentrate the electrical field during electroporation, and therefore maximize its effect on cell membrane permeability. So far, the methods used for the fabrication of MN electrodes have been relatively limited with respect to the needle design. In this work, we provide a method to fabricate MNs using 3D printing, which is a technology that provides a high degree of flexibility with respect to geometry and dimensions. Pyramidal-shaped MN designs were fabricated and tested on HCT116 cancer cells. Customization of the tips of the pyramids permits tailoring of the electrical field in the vicinity of the cell membranes. The fabricated device enables low-voltage (2 V) electroporation, eliminating the need for the use of specialized chemical buffers. The results show the potential of this method, which can be exploited and optimized for many different applications, and offer a very accessible approach for in-vitro electroporation and cell studies. The MNs can be customized to create complex structures, for example, for a multi-culture cell environment.
    Citation
    Moussi, K., Kavaldzhiev, M., Perez, J. E., Alsharif, N., Merzaban, J., & Kosel, J. (2020). 3D Printed Microneedle Array for Electroporation. 2020 42nd Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC). doi:10.1109/embc44109.2020.9175748
    Publisher
    IEEE
    Conference/Event name
    2020 42nd Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC)
    ISBN
    978-1-7281-1991-5
    DOI
    10.1109/EMBC44109.2020.9175748
    Additional Links
    https://ieeexplore.ieee.org/document/9175748/
    https://ieeexplore.ieee.org/document/9175748/
    https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=9175748
    ae974a485f413a2113503eed53cd6c53
    10.1109/EMBC44109.2020.9175748
    Scopus Count
    Collections
    Conference Papers; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Physical Science and Engineering (PSE) Division; Electrical Engineering Program; Chemical Engineering Program; Sensing, Magnetism and Microsystems Lab; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

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