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3D Printed Microneedle Array for Electroporation.pdf
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3.666Mb
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Description:
Accepted manuscript
Type
Conference PaperAuthors
Moussi, Khalil
Kavaldzhiev, Mincho

Perez, Jose E.

Alsharif, Nouf

Merzaban, Jasmeen

Kosel, Jürgen

KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Chemical Engineering Program
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Electrical Engineering Program
Physical Science and Engineering (PSE) Division
Sensing, Magnetism and Microsystems Lab
Date
2020-08-28Online Publication Date
2020-08-28Print Publication Date
2020-07Permanent link to this record
http://hdl.handle.net/10754/665215
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Show full item recordAbstract
In-vitro transfection of cells by electroporation is a widely used approach in cell biology and medicine. The transfection method is highly dependent on the cell culture’s electrical resistance, which is strongly determined by differences in the membranes, but also on the morphology of the electrodes. Microneedle (MN)-based electrodes have been used to concentrate the electrical field during electroporation, and therefore maximize its effect on cell membrane permeability. So far, the methods used for the fabrication of MN electrodes have been relatively limited with respect to the needle design. In this work, we provide a method to fabricate MNs using 3D printing, which is a technology that provides a high degree of flexibility with respect to geometry and dimensions. Pyramidal-shaped MN designs were fabricated and tested on HCT116 cancer cells. Customization of the tips of the pyramids permits tailoring of the electrical field in the vicinity of the cell membranes. The fabricated device enables low-voltage (2 V) electroporation, eliminating the need for the use of specialized chemical buffers. The results show the potential of this method, which can be exploited and optimized for many different applications, and offer a very accessible approach for in-vitro electroporation and cell studies. The MNs can be customized to create complex structures, for example, for a multi-culture cell environment.Citation
Moussi, K., Kavaldzhiev, M., Perez, J. E., Alsharif, N., Merzaban, J., & Kosel, J. (2020). 3D Printed Microneedle Array for Electroporation. 2020 42nd Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC). doi:10.1109/embc44109.2020.9175748Publisher
IEEEConference/Event name
2020 42nd Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC)ISBN
978-1-7281-1991-5Additional Links
https://ieeexplore.ieee.org/document/9175748/https://ieeexplore.ieee.org/document/9175748/
https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=9175748
ae974a485f413a2113503eed53cd6c53
10.1109/EMBC44109.2020.9175748