Perez, Jose E.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Chemical Engineering Program
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Electrical Engineering Program
Physical Science and Engineering (PSE) Division
Sensing, Magnetism and Microsystems Lab
Online Publication Date2020-08-28
Print Publication Date2020-07
Permanent link to this recordhttp://hdl.handle.net/10754/665215
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AbstractIn-vitro transfection of cells by electroporation is a widely used approach in cell biology and medicine. The transfection method is highly dependent on the cell culture’s electrical resistance, which is strongly determined by differences in the membranes, but also on the morphology of the electrodes. Microneedle (MN)-based electrodes have been used to concentrate the electrical field during electroporation, and therefore maximize its effect on cell membrane permeability. So far, the methods used for the fabrication of MN electrodes have been relatively limited with respect to the needle design. In this work, we provide a method to fabricate MNs using 3D printing, which is a technology that provides a high degree of flexibility with respect to geometry and dimensions. Pyramidal-shaped MN designs were fabricated and tested on HCT116 cancer cells. Customization of the tips of the pyramids permits tailoring of the electrical field in the vicinity of the cell membranes. The fabricated device enables low-voltage (2 V) electroporation, eliminating the need for the use of specialized chemical buffers. The results show the potential of this method, which can be exploited and optimized for many different applications, and offer a very accessible approach for in-vitro electroporation and cell studies. The MNs can be customized to create complex structures, for example, for a multi-culture cell environment.
CitationMoussi, K., Kavaldzhiev, M., Perez, J. E., Alsharif, N., Merzaban, J., & Kosel, J. (2020). 3D Printed Microneedle Array for Electroporation. 2020 42nd Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC). doi:10.1109/embc44109.2020.9175748
Conference/Event name2020 42nd Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC)