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dc.contributor.authorMoussi, Khalil
dc.contributor.authorAbu Samra, Dina Bashir Kamil
dc.contributor.authorYassine, Omar
dc.contributor.authorMerzaban, Jasmeen
dc.contributor.authorKosel, Jürgen
dc.date.accessioned2020-09-17T11:22:13Z
dc.date.available2020-09-17T11:22:13Z
dc.date.issued2020-08-28
dc.identifier.citationMoussi, K., Abusamra, D. B., Yassine, O., Merzaban, J., & Kosel, J. (2020). Strain-induced Differentiation of Mesenchymal Stem Cells. 2020 42nd Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC). doi:10.1109/embc44109.2020.9176273
dc.identifier.isbn978-1-7281-1991-5
dc.identifier.issn1557-170X
dc.identifier.doi10.1109/EMBC44109.2020.9176273
dc.identifier.urihttp://hdl.handle.net/10754/665214
dc.description.abstractDirecting the fate of human mesenchymal stem/stromal cells (hMSCs) toward bone formation using mechanical strain is a promising approach in regenerative medicine related to bone diseases. Numerous studies have evaluated the effects of vibration or cyclic tensile strain on MSCs towards developing a mechanically-based method for stimulating differentiation. Here, we study the differentiation of hMSCs cultured on elastic polydimethylsiloxane (PDMS) membrane, which is magnetically actuated to induce periodically varying strain. The strain distribution across the membrane was calculated by finite-element modeling and demonstrates three main areas of different strain amplitudes. The strain effect on the hMSCs was evaluated by measuring the mineralization of differentiated hMSCs using Alizarin S red stain. The results indicate a strain-dependent differentiation of hMSCs, where the highest region of strain on the membrane resulted in the most accelerated differentiation. Osteogenic differentiation was achieved as early as two weeks, which is significantly sooner than control hMSCs treated with osteogenic media alone.
dc.publisherInstitute of Electrical and Electronics Engineers (IEEE)
dc.relation.urlhttps://ieeexplore.ieee.org/document/9176273/
dc.relation.urlhttps://ieeexplore.ieee.org/document/9176273/
dc.relation.urlhttps://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=9176273
dc.rightsArchived with thanks to IEEE
dc.subjectMesenchymal stem cells
dc.subjectMechanical strain
dc.subjectOsteogenic differentiation
dc.titleStrain-induced Differentiation of Mesenchymal Stem Cells
dc.typeConference Paper
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentChemical Engineering Program
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.contributor.departmentElectrical Engineering Program
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.contributor.departmentSensing, Magnetism and Microsystems Lab
dc.conference.date20-24 July 2020
dc.conference.name2020 42nd Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC)
dc.conference.locationMontreal, QC, Canada
dc.eprint.versionPost-print
kaust.personMoussi, Khalil
kaust.personAbu Samra, Dina Bashir Kamil
kaust.personYassine, Omar
kaust.personMerzaban, Jasmeen S.
kaust.personKosel, Jürgen
refterms.dateFOA2020-09-17T13:54:18Z
dc.date.published-online2020-08-28
dc.date.published-print2020-07


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