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    Using Eukaryotic Expression Systems to Generate Human α1,3 Fucosyltransferases that Effectively Create Selectin-binding Glycans on Stem Cells

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    Using-using eukaryotic.pdf
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    Type
    Article
    Authors
    Al-Amoodi, Asma S. cc
    Sakashita, Kosuke cc
    Ali, Amal J. cc
    Zhou, Ruoyu
    Lee, Jae Man
    Tehseen, Muhammad cc
    Li, Mo cc
    Izpisua Belmonte, Juan Carlos
    Kusakabe, Takahiro
    Merzaban, Jasmeen cc
    KAUST Department
    Bioscience Program
    Biological and Environmental Sciences and Engineering (BESE) Division
    Proteomics and Protein Expression
    Date
    2020-09-09
    Online Publication Date
    2020-09-09
    Print Publication Date
    2020-10-06
    Embargo End Date
    2021-09-09
    Permanent link to this record
    http://hdl.handle.net/10754/665213
    
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    Abstract
    Recruitment of circulating cells towards target sites is primarily dependent on selectin/ligand adhesive interactions.Glycosyltransferases are involved in the creation of selectin ligands on proteins and lipids. α1,3-fucosylation is imperative for the creation of selectin ligands and a number of fucosyltransferases (FTs) are able to modify terminal lactosamines on cells to create these ligands. One FT, fucosyltransferase VI (FTVI) adds a fucose in an α1,3 configuration to N-acetylglucosamine to generate sialyl Lewis X (sLex) epitopes on proteins of live cells and enhances their ability to bind E-selectin. Although a number of recombinant human FTVIs have been purified, apart from limited commercial enzymes, they were not characterized for their activity on live cells. Here we focused on establishing a robust method to produce FTVI that is active on living cells (hematopoietic cells and mesenchymal stromal cells). To this end, we used two expression systems, Bombyx mori (silkworm) and Pichia pastoris (yeast), to produce significant amounts of N-terminally tagged FTVI and demonstrated that these enzymes have superior activity when compared to currently available commercial enzymes that are produced from various expression systems. Overall, we outline a scheme to attain large amounts of highly active FTVI that can be used for the applications of FTVI in enhancing the engraftment of cells lacking the sLex epitopes.
    Citation
    Al-Amoodi, A. S., Sakashita, K., Ali, A. J., Zhou, R., Lee, J. M., Tehseen, M., … Merzaban, J. S. (2020). Using Eukaryotic Expression Systems to Generate Human α1,3 Fucosyltransferases that Effectively Create Selectin-binding Glycans on Stem Cells. Biochemistry. doi:10.1021/acs.biochem.0c00523
    Publisher
    American Chemical Society (ACS)
    Journal
    Biochemistry
    DOI
    10.1021/acs.biochem.0c00523
    Additional Links
    https://pubs.acs.org/doi/10.1021/acs.biochem.0c00523
    ae974a485f413a2113503eed53cd6c53
    10.1021/acs.biochem.0c00523
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program

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