First report of Klebsiella quasipneumoniae harboring blaKPC-2 in Saudi Arabia
Antony, Chakkiath Paul
Althaqafi, Abdulhakeem O.
Alhaj-Hussein, Baraa T.
Zowawi, Hosam M.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Pathogen Genomics Laboratory
Red Sea Research Center (RSRC)
Permanent link to this recordhttp://hdl.handle.net/10754/665167
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AbstractAbstract Background Nosocomial infections caused by multi-drug resistant Enterobacteriaceae are a global public health threat that ought to be promptly identified, reported, and addressed accurately. Many carbapenem-resistant Enterobacteriaceae-associated genes have been identified in Saudi Arabia but not the endemic Klebsiella pneumoniae carbapenemases (KPCs), which are encoded by blaKPC-type genes. KPCs are known for their exceptional spreading potential. Methods We collected n = 286 multi-drug resistant (MDR) Klebsiella spp. isolates as part of screening for resistant patterns from a tertiary hospital in Saudi Arabia between 2014 and 2018. Antimicrobial susceptibility testing was carried out using both VITEK II and the broth microdilution of all collected isolates. Detection of resistance-conferring genes was carried out using Illumina whole-genome shotgun sequencing and PacBio SMRT sequencing protocols. Results A Carbapenem-resistant Enterobacteriaceae (CRE) Klebsiella quasipneumoniae subsp. similipneumoniae strain was identified as a novel ST-3510 carrying a blaKPC-2 carbapenemase encoding gene. The isolate, designated as NGKPC-421, was obtained from shotgun Whole Genome Sequencing (WGS) surveillance of 286 MDR Klebsiella spp. clinical isolates. The NGKPC-421 isolate was collected from a septic patient in late 2017 and was initially misidentified as K. pneumoniae. The sequencing and assembly of the NGKPC-421 genome resulted in the identification of a putative ~ 39.4 kb IncX6 plasmid harboring a blaKPC-2 gene, flanked by transposable elements (ISKpn6-blaKPC-2–ISKpn27). Conclusion This is the first identification of a KPC-2-producing CRE in the Gulf region. The impact on this finding is of major concern to the public health in Saudi Arabia, considering that it is the religious epicenter with a continuous mass influx of pilgrims from across the world. Our study strongly highlights the importance of implementing rapid sequencing-based technologies in clinical microbiology for precise taxonomic classification and monitoring of antimicrobial resistance patterns.
CitationHala, S., Chakkiath Antony, Alshehri, M., Abdulhakeem Althaqafi, Alsaedi, A., Areej Mufti, Kaaki, M., Baraa Alhaj-Hussein, Hosam Zowawi, Abdulfattah Al-Amri, & Arnab Pain. (2019). First report of Klebsiella quasipneumoniae harboring blaKPC-2 in Saudi Arabia. figshare. https://doi.org/10.6084/M9.FIGSHARE.C.4792179
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Hala, S., Antony, C. P., Alshehri, M., Althaqafi, A. O., Alsaedi, A., Mufti, A., … Pain, A. (2019). First report of Klebsiella quasipneumoniae harboring blaKPC-2 in Saudi Arabia. Antimicrobial Resistance & Infection Control, 8(1). doi:10.1186/s13756-019-0653-9. DOI: 10.1186/s13756-019-0653-9 HANDLE: 10754/660869
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