Plasmodium vinckei genomes provide insights into the pan-genome and evolution of rodent malaria parasites.
KAUST DepartmentBiological and Environmental Science and Engineering (BESE) Division
Pathogen Genomics Laboratory
Online Publication Date2021-04-23
Print Publication Date2021-12
Permanent link to this recordhttp://hdl.handle.net/10754/665129
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AbstractBackgroundRodent malaria parasites (RMPs) serve as tractable tools to study malaria parasite biology and host-parasite-vector interactions. Among the four RMPs originally collected from wild thicket rats in sub-Saharan Central Africa and adapted to laboratory mice, Plasmodium vinckei is the most geographically widespread with isolates collected from five separate locations. However, there is a lack of extensive phenotype and genotype data associated with this species, thus hindering its use in experimental studies.ResultsWe have generated a comprehensive genetic resource for P. vinckei comprising of five reference-quality genomes, one for each of its subspecies, blood-stage RNA sequencing data for five P. vinckei isolates, and genotypes and growth phenotypes for ten isolates. Additionally, we sequenced seven isolates of the RMP species Plasmodium chabaudi and Plasmodium yoelii, thus extending genotypic information for four additional subspecies enabling a re-evaluation of the genotypic diversity and evolutionary history of RMPs. The five subspecies of P. vinckei have diverged widely from their common ancestor and have undergone large-scale genome rearrangements. Comparing P. vinckei genotypes reveals region-specific selection pressures particularly on genes involved in mosquito transmission. Using phylogenetic analyses, we show that RMP multigene families have evolved differently across the vinckei and berghei groups of RMPs and that family-specific expansions in P. chabaudi and P. vinckei occurred in the common vinckei group ancestor prior to speciation. The erythrocyte membrane antigen 1 and fam-c families in particular show considerable expansions among the lowland forest-dwelling P. vinckei parasites. The subspecies from the highland forests of Katanga, P. v. vinckei, has a uniquely smaller genome, a reduced multigene family repertoire and is also amenable to transfection making it an ideal parasite for reverse genetics. We also show that P. vinckei parasites are amenable to genetic crosses.ConclusionsPlasmodium vinckei isolates display a large degree of phenotypic and genotypic diversity and could serve as a resource to study parasite virulence and immunogenicity. Inclusion of P. vinckei genomes provide new insights into the evolution of RMPs and their multigene families. Amenability to genetic crossing and transfection make them also suitable for classical and functional genetics to study Plasmodium biology.
CitationRamaprasad, A., Klaus, S., Culleton, R., & Pain, A. (2020). Plasmodium vinckei genomes provide insights into the pan-genome and evolution of rodent malaria parasites. doi:10.1101/2020.09.07.286369
SponsorsThe authors would like to acknowledge the personnel at the Bioscience Core Laboratory (BCL) at King Abdullah University of Science and Technology for their help with next-generation sequencing. We acknowledge the contribution of Professor Richard Carter, whose support of this work was crucial to its inception and completion.
RC was supported by a Grant (JP16K21233) from the Japan Society for the Promotion of Science. This work was supported by KAUST faculty baseline fund (BAS/1/1020-01-01) and Competitive Research Fund (URF/1/2267-01-01) to AP.
PublisherCold Spring Harbor Laboratory
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