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dc.contributor.authorAragon-Raygoza, Alejandro
dc.contributor.authorVasco, Alejandra
dc.contributor.authorBlilou, Ikram
dc.contributor.authorHerrera-Estrella, Luis Rafael
dc.contributor.authorCruz-Ramírez, Alfredo
dc.date.accessioned2020-09-14T08:12:29Z
dc.date.available2020-09-14T08:12:29Z
dc.date.issued2020-12-09
dc.date.submitted2020-10-27
dc.identifier.citationAragón-Raygoza, A., Vasco, A., Blilou, I., Herrera-Estrella, L., & Cruz-Ramírez, A. (2020). Development and Cell Cycle Activity of the Root Apical Meristem in the Fern Ceratopteris richardii. Genes, 11(12), 1455. doi:10.3390/genes11121455
dc.identifier.issn2073-4425
dc.identifier.pmid33291610
dc.identifier.doi10.3390/genes11121455
dc.identifier.doi10.1101/2020.08.27.271049
dc.identifier.urihttp://hdl.handle.net/10754/665116
dc.description.abstractFerns are a representative clade in plant evolution although underestimated in the genomic era. Ceratopteris richardii is an emergent model for developmental processes in ferns, yet a complete scheme of the different growth stages is necessary. Here, we present a developmental analysis, at the tissue and cellular levels, of the first shoot-borne root of Ceratopteris. We followed early stages and emergence of the root meristem in sporelings. While assessing root growth, the first shoot-borne root ceases its elongation between the emergence of the fifth and sixth roots, suggesting Ceratopteris roots follow a determinate developmental program. We report cell division frequencies in the stem cell niche after detecting labeled nuclei in the root apical cell (RAC) and derivatives after 8 h of exposure. These results demonstrate the RAC has a continuous mitotic activity during root development. Detection of cell cycle activity in the RAC at early times suggests this cell acts as a non-quiescent organizing center. Overall, our results provide a framework to study root function and development in ferns and to better understand the evolutionary history of this organ.
dc.description.sponsorshipThis research received no external funding. A.A.-R. was financially supported by a CONACyT PhD Fellowship (No. 421596). We thank Jane Langdale and Andrew Plackett (Oxford University), and Pamela Soltis, Doug Soltis and Blaine Marchant (University of Florida), for providing us with Ceratopteris richardii Hn-n spores. To our lab manager Annie Espinal-Centeno for obtaining the first batch of Ceratopteris spores to begin with this project.
dc.publisherMDPI AG
dc.relation.urlhttps://www.mdpi.com/2073-4425/11/12/1455
dc.relation.urlhttp://biorxiv.org/lookup/doi/10.1101/2020.08.27.271049
dc.rightsThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleDevelopment and Cell Cycle Activity of the Root Apical Meristem in the Fern Ceratopteris richardii
dc.typeArticle
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentCenter for Desert Agriculture
dc.contributor.departmentPlant Science
dc.identifier.journalGenes
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionMolecular and Developmental Complexity Group at Unidad de Genómica Avanzada, Laboratorio Nacional de Genómica para la Biodiversidad, Cinvestav Sede Irapuato, Km. 9.6 Libramiento Norte Carretera, Irapuato-León, Irapuato, 36821, Guanajuato, Mexico;
dc.contributor.institutionMetabolic Engineering Group, Unidad de Genómica Avanzada, Laboratorio Nacional de Genómica para la Biodiversidad, Cinvestav Sede Irapuato, Km. 9.6 Libramiento Norte Carretera, Irapuato-León, Irapuato, 36821, Guanajuato, Mexico
dc.contributor.institutionBotanical Research Institute of Texas (BRIT), Fort Worth, TX, 76107-3400, USA;
dc.contributor.institutionInstitute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Science, Texas Tech University, Lubbock, TX, 79409, USA
dc.identifier.volume11
dc.identifier.issue12
dc.identifier.pages1455
kaust.personBlilou, Ikram
dc.date.accepted2020-11-26
dc.identifier.eid2-s2.0-85097243700
refterms.dateFOA2020-09-14T08:13:01Z


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This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
Except where otherwise noted, this item's license is described as This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.