Development and Cell Cycle Activity of the Root Apical Meristem in the Fern Ceratopteris richardii
Herrera-Estrella, Luis Rafael
KAUST DepartmentBiological and Environmental Science and Engineering (BESE) Division
Center for Desert Agriculture
Permanent link to this recordhttp://hdl.handle.net/10754/665116
MetadataShow full item record
AbstractFerns are a representative clade in plant evolution although underestimated in the genomic era. Ceratopteris richardii is an emergent model for developmental processes in ferns, yet a complete scheme of the different growth stages is necessary. Here, we present a developmental analysis, at the tissue and cellular levels, of the first shoot-borne root of Ceratopteris. We followed early stages and emergence of the root meristem in sporelings. While assessing root growth, the first shoot-borne root ceases its elongation between the emergence of the fifth and sixth roots, suggesting Ceratopteris roots follow a determinate developmental program. We report cell division frequencies in the stem cell niche after detecting labeled nuclei in the root apical cell (RAC) and derivatives after 8 h of exposure. These results demonstrate the RAC has a continuous mitotic activity during root development. Detection of cell cycle activity in the RAC at early times suggests this cell acts as a non-quiescent organizing center. Overall, our results provide a framework to study root function and development in ferns and to better understand the evolutionary history of this organ.
CitationAragón-Raygoza, A., Vasco, A., Blilou, I., Herrera-Estrella, L., & Cruz-Ramírez, A. (2020). Development and Cell Cycle Activity of the Root Apical Meristem in the Fern Ceratopteris richardii. Genes, 11(12), 1455. doi:10.3390/genes11121455
SponsorsThis research received no external funding. A.A.-R. was financially supported by a CONACyT PhD Fellowship (No. 421596). We thank Jane Langdale and Andrew Plackett (Oxford University), and Pamela Soltis, Doug Soltis and Blaine Marchant (University of Florida), for providing us with Ceratopteris richardii Hn-n spores. To our lab manager Annie Espinal-Centeno for obtaining the first batch of Ceratopteris spores to begin with this project.
Except where otherwise noted, this item's license is described as This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
- Meristem development and activity in gametophytes of the model fern, Ceratopteris richardii.
- Authors: Bartz M, Gola EM
- Issue date: 2018 Dec 15
- <i>LEAFY</i> maintains apical stem cell activity during shoot development in the fern <i>Ceratopteris richardii</i>.
- Authors: Plackett AR, Conway SJ, Hewett Hazelton KD, Rabbinowitsch EH, Langdale JA, Di Stilio VS
- Issue date: 2018 Oct 24
- Developmental anatomy and auxin response of lateral root formation in Ceratopteris richardii.
- Authors: Hou G, Hill JP, Blancaflor EB
- Issue date: 2004 Mar
- Vascular function of the T3/modern clade WUSCHEL-Related HOMEOBOX transcription factor genes predate apical meristem-maintenance function.
- Authors: Youngstrom CE, Withers KA, Irish EE, Cheng CL
- Issue date: 2022 Apr 25
- Developmental anatomy of the fifth shoot-borne root in young sporophytes of Ceratopteris richardii.
- Authors: Hou GC, Hill JP
- Issue date: 2004 Jun