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dc.contributor.authorOrtega, Alejandra
dc.contributor.authorGeraldi, Nathan
dc.contributor.authorDuarte, Carlos M.
dc.date.accessioned2020-09-09T12:17:40Z
dc.date.available2020-09-09T12:17:40Z
dc.date.issued2020-08-31
dc.date.submitted2019-10-15
dc.identifier.citationOrtega, A., Geraldi, N. R., & Duarte, C. M. (2020). Environmental DNA identifies marine macrophyte contributions to Blue Carbon sediments. Limnology and Oceanography. doi:10.1002/lno.11579
dc.identifier.issn1939-5590
dc.identifier.doi10.1002/lno.11579
dc.identifier.urihttp://hdl.handle.net/10754/665040
dc.description.abstractEstimation of marine macrophyte contribution to coastal sediments is key to understand carbon sequestration dynamics. Nevertheless, identification of macrophyte carbon is challenging. We propose environmental DNA (eDNA) metabarcoding as a new approach for identification of sediment contributors, and compared this approach against stable isotopes—the traditional approach. eDNA metabarcoding allowed high-resolution identification of 48 macroalgae, seagrasses, and mangroves from coastal habitats. The relative eDNA contributions of macrophytes were similar to their contributions of organic carbon based on stable isotopes; however, isotopes were unreliable for taxonomical discrimination among macrophyte sources. Additionally, we experimentally found that eDNA abundance in the sediment correlates with both the DNA (84%, R2 = 0.71, p = 0.001) and the organic carbon content (76%, R2 = 0.58, p = 0.006) per macrophyte lineage. These results demonstrate the unparallel resolution of eDNA as a method for estimation of the organic carbon contribution of marine macrophytes to blue carbon stocks.
dc.description.sponsorshipThis research was funded by King Abdullah University of Science and Technology through baseline funding provided to CMD. We thank CMOR staff, R/V Thuwal crew, Andrea Anton, and members of CMD research group for help during sampling. We also thank Rubén Díaz-Rúa and Wajitha J. Raja Mohamed Sait for their help in sample processing.
dc.publisherWiley
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/abs/10.1002/lno.11579
dc.rightsThis is an open access article under the terms of the Creative CommonsAttribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleEnvironmental DNA identifies marine macrophyte contributions to Blue Carbon sediments
dc.typeArticle
dc.contributor.departmentBioscience Program
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.contributor.departmentMarine Science Program
dc.identifier.journalLimnology and Oceanography
dc.eprint.versionPublisher's Version/PDF
kaust.personOrtega, Alejandra
kaust.personGeraldi, Nathan
kaust.personDuarte, Carlos M.
dc.date.accepted2020-07-25
dc.identifier.eid2-s2.0-85089963357
refterms.dateFOA2020-09-09T12:18:06Z


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This is an open access article under the terms of the Creative CommonsAttribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as This is an open access article under the terms of the Creative CommonsAttribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.