Long-read individual-molecule sequencing reveals CRISPR-induced genetic heterogeneity in human ESCs
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Computational Bioscience Research Center (CBRC)
Computer Science Program
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Laboratory of Stem Cell and Regeneration, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia.
Structural and Functional Bioinformatics Group
Permanent link to this recordhttp://hdl.handle.net/10754/664976
MetadataShow full item record
AbstractAbstract Quantifying the genetic heterogeneity of a cell population is essential to understanding of biological systems. We develop a universal method to label individual DNA molecules for single-base-resolution haplotype-resolved quantitative characterization of diverse types of rare variants, with frequency as low as 4 × 10−5, using both short- or long-read sequencing platforms. It provides the first quantitative evidence of persistent nonrandom large structural variants and an increase in single-nucleotide variants at the on-target locus following repair of double-strand breaks induced by CRISPR-Cas9 in human embryonic stem cells.
CitationChongwei Bi, Wang, L., Baolei Yuan, Zhou, X., Li, Y., Wang, S., Yuhong Pang, Gao, X., Yanyi Huang, & Li, M. (2020). Long-read individual-molecule sequencing reveals CRISPR-induced genetic heterogeneity in human ESCs. figshare. https://doi.org/10.6084/M9.FIGSHARE.C.5100908
RelationsIs Supplement To: