Show simple item record

dc.contributor.authorSanz-Sáez, Isabel
dc.contributor.authorSalazar, Guillem
dc.contributor.authorSánchez, Pablo
dc.contributor.authorLara, Elena
dc.contributor.authorRoyo-Llonch, Marta
dc.contributor.authorSà, Elisabet L.
dc.contributor.authorLucena, Teresa
dc.contributor.authorPujalte, María J.
dc.contributor.authorVaqué, Dolors
dc.contributor.authorDuarte, Carlos M.
dc.contributor.authorGasol, Josep M.
dc.contributor.authorPedrós-Alió, Carlos
dc.contributor.authorSánchez, Olga
dc.contributor.authorAcinas, Silvia G.
dc.date.accessioned2020-09-07T08:40:38Z
dc.date.available2020-09-07T08:40:38Z
dc.date.issued2020
dc.identifier.citationSanz-Sáez, I., Salazar, G., Sánchez, P., Lara, E., Royo-Llonch, M., Sà, E. L., Lucena, T., Pujalte, M. J., Vaqué, D., Duarte, C. M., Gasol, J. M., Pedrós-Alió, C., Sánchez, O., & Acinas, S. G. (2020). Diversity and distribution of marine heterotrophic bacteria from a large culture collection. figshare. https://doi.org/10.6084/M9.FIGSHARE.C.5058382.V1
dc.identifier.doi10.6084/m9.figshare.c.5058382.v1
dc.identifier.urihttp://hdl.handle.net/10754/664970
dc.description.abstractAbstract Background Isolation of marine microorganisms is fundamental to gather information about their physiology, ecology and genomic content. To date, most of the bacterial isolation efforts have focused on the photic ocean leaving the deep ocean less explored. We have created a marine culture collection of heterotrophic bacteria (MARINHET) using a standard marine medium comprising a total of 1561 bacterial strains, and covering a variety of oceanographic regions from different seasons and years, from 2009 to 2015. Specifically, our marine collection contains isolates from both photic (817) and aphotic layers (744), including the mesopelagic (362) and the bathypelagic (382), from the North Western Mediterranean Sea, the North and South Atlantic Ocean, the Indian, the Pacific, and the Arctic Oceans. We described the taxonomy, the phylogenetic diversity and the biogeography of a fraction of the marine culturable microorganisms to enhance our knowledge about which heterotrophic marine isolates are recurrently retrieved across oceans and along different depths. Results The partial sequencing of the 16S rRNA gene of all isolates revealed that they mainly affiliate with the classes Alphaproteobacteria (35.9%), Gammaproteobacteria (38.6%), and phylum Bacteroidetes (16.5%). In addition, Alteromonas and Erythrobacter genera were found the most common heterotrophic bacteria in the ocean growing in solid agar medium. When comparing all photic, mesopelagic, and bathypelagic isolates sequences retrieved from different stations, 37% of them were 100% identical. This percentage increased up to 59% when mesopelagic and bathypelagic strains were grouped as the aphotic dataset and compared to the photic dataset of isolates, indicating the ubiquity of some bacterial isolates along different ocean depths. Finally, we isolated three strains that represent a new species, and the genome comparison and phenotypic characterization of two of these strains (ISS653 and ISS1889) concluded that they belong to a new species within the genus Mesonia. Conclusions Overall, this study highlights the relevance of culture-dependent studies, with focus on marine isolated bacteria from different oceanographic regions and depths, to provide a more comprehensive view of the culturable marine bacteria as part of the total marine microbial diversity.
dc.publisherfigshare
dc.subjectMicrobiology
dc.subjectFOS: Biological sciences
dc.subjectOceanography
dc.subjectFOS: Earth and related environmental sciences
dc.titleDiversity and distribution of marine heterotrophic bacteria from a large culture collection
dc.typeDataset
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentMarine Science Program
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.contributor.institutionDepartment of Marine Biology and Oceanography, Institut de Ciències del Mar (CSIC), 08003 Barcelona, Spain.
dc.contributor.institutionDepartment of Biology, Institute of Microbiology, ETH Zurich, Vladimir-Prelog-Weg 1-5/10, CH-8093 Zurich, Switzerland.
dc.contributor.institutionInstitute of Marine Sciences (CNR-ISMAR), National Research Council, Castello 2737/F Arsenale-Tesa 104, 30122 Venezia, Italy.
dc.contributor.institutionDepartamento de Microbiología y Ecología and Colección Española de Cultivos Tipo (CECT), Universitat de València, Valencia, Spain.
dc.contributor.institutionDepartment of Systems Biology, Centro Nacional de Biotecnología (CNB), CSIC, Madrid, Spain.
dc.contributor.institutionDepartament de Genètica i Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
kaust.personDuarte, Carlos M.
kaust.personDuarte, Carlos M.
dc.relation.issupplementtoDOI:10.1186/s12866-020-01884-7
display.relations<b> Is Supplement To:</b><br/> <ul> <li><i>[Article]</i> <br/> Sanz-Sáez, I., Salazar, G., Sánchez, P., Lara, E., Royo-Llonch, M., Sà, E. L., … Acinas, S. G. (2020). Diversity and distribution of marine heterotrophic bacteria from a large culture collection. BMC Microbiology, 20(1). doi:10.1186/s12866-020-01884-7. DOI: <a href="https://doi.org/10.1186/s12866-020-01884-7" >10.1186/s12866-020-01884-7</a> HANDLE: <a href="http://hdl.handle.net/10754/664251">10754/664251</a></li></ul>


This item appears in the following Collection(s)

Show simple item record