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dc.contributor.authorSuen, Kin Man
dc.contributor.authorBraukmann, Fabian
dc.contributor.authorButler, Richard
dc.contributor.authorBensaddek, Dalila
dc.contributor.authorAkay, Alper
dc.contributor.authorLin, Chi-Chuan
dc.contributor.authorMilonaitytė, Dovilė
dc.contributor.authorDoshi, Neel
dc.contributor.authorSapetschnig, Alexandra
dc.contributor.authorLamond, Angus
dc.contributor.authorLadbury, John Edward
dc.contributor.authorMiska, Eric Alexander
dc.date.accessioned2020-09-06T08:55:01Z
dc.date.available2020-09-06T08:55:01Z
dc.date.issued2020-08-25
dc.date.submitted2019-05-22
dc.identifier.citationSuen, K. M., Braukmann, F., Butler, R., Bensaddek, D., Akay, A., Lin, C.-C., … Miska, E. A. (2020). DEPS-1 is required for piRNA-dependent silencing and PIWI condensate organisation in Caenorhabditis elegans. Nature Communications, 11(1). doi:10.1038/s41467-020-18089-1
dc.identifier.issn2041-1723
dc.identifier.pmid32843637
dc.identifier.doi10.1038/s41467-020-18089-1
dc.identifier.urihttp://hdl.handle.net/10754/664937
dc.description.abstractMembraneless organelles are sites for RNA biology including small non-coding RNA (ncRNA) mediated gene silencing. How small ncRNAs utilise phase separated environments for their function is unclear. We investigated how the PIWI-interacting RNA (piRNA) pathway engages with the membraneless organelle P granule in Caenorhabditis elegans. Proteomic analysis of the PIWI protein PRG-1 reveals an interaction with the constitutive P granule protein DEPS-1. DEPS-1 is not required for piRNA biogenesis but piRNA-dependent silencing: deps-1 mutants fail to produce the secondary endo-siRNAs required for the silencing of piRNA targets. We identify a motif on DEPS-1 which mediates a direct interaction with PRG-1. DEPS-1 and PRG-1 form intertwining clusters to build elongated condensates in vivo which are dependent on the Piwi-interacting motif of DEPS-1. Additionally, we identify EDG-1 as an interactor of DEPS-1 and PRG-1. Our study reveals how specific protein-protein interactions drive the spatial organisation and piRNA-dependent silencing within membraneless organelles.
dc.description.sponsorshipWe are grateful for the generous gifts of the DEPS-1
dc.publisherSpringer Science and Business Media LLC
dc.relation.urlhttp://www.nature.com/articles/s41467-020-18089-1
dc.rightsThis article is open access licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleDEPS-1 is required for piRNA-dependent silencing and PIWI condensate organisation in Caenorhabditis elegans.
dc.typeArticle
dc.contributor.departmentProteomics and Protein Expression
dc.identifier.journalNature communications
dc.identifier.pmcidPMC7447803
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionWellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QN, UK
dc.contributor.institutionDepartment of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK
dc.contributor.institutionLaboratory for Quantitative Proteomics, Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, UK
dc.contributor.institutionSchool of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK
dc.contributor.institutionSchool of Molecular and Cellular Biology, University of Leeds, LC Miall Building, Leeds, LS2 9JT, UK
dc.contributor.institutionUniversity of Cambridge, School of Clinical Medicine, Cambridge Biomedical Campus, Cambridge, CB2 0SP, UK
dc.contributor.institutionWellcome Sanger Institute, Wellcome Trust Genome Campus, Hinxton, CB10 1SA, UK
dc.identifier.volume11
dc.identifier.issue1
kaust.personBensaddek, Dalila
dc.date.accepted2020-07-18
dc.identifier.eid2-s2.0-85089771119
refterms.dateFOA2020-09-06T08:55:55Z
dc.date.published-online2020-08-25
dc.date.published-print2020-12


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This article is open access licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder
Except where otherwise noted, this item's license is described as This article is open access licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder