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dc.contributor.authorRchiad, ‍Zineb
dc.contributor.authorHaidar, Malak
dc.contributor.authorAnsari, Hifzur Rahman
dc.contributor.authorTajeri, Shahin
dc.contributor.authorMfarrej, Sara
dc.contributor.authorBen Rached, Fathia
dc.contributor.authorKaushik, Abhinav
dc.contributor.authorLangsley, Gordon
dc.contributor.authorPain, Arnab
dc.date.accessioned2020-08-25T06:18:50Z
dc.date.available2020-08-25T06:18:50Z
dc.date.issued2020-09-29
dc.date.submitted2020-06-09
dc.identifier.citationRchiad, Z., Haidar, M., Ansari, H. R., Tajeri, S., Mfarrej, S., Ben Rached, F., … Pain, A. (2020). Novel tumour suppressor roles for GZMA and RASGRP1 in Theileria annulata -transformed macrophages and human B-lymphoma cells. Cellular Microbiology. doi:10.1111/cmi.13255
dc.identifier.issn1462-5814
dc.identifier.issn1462-5822
dc.identifier.doi10.1111/cmi.13255
dc.identifier.urihttp://hdl.handle.net/10754/664809
dc.description.abstractTheileria annulata is a tick-transmitted apicomplexan parasite that infects and transforms bovine leukocytes into disseminating tumours that cause a disease called tropical theileriosis. Using comparative transcriptomics we identified genes transcriptionally perturbed during Theileria-induced leukocyte transformation. Dataset comparisons highlighted a small set of genes associated with Theileria-transformed leukocyte dissemination. The roles of Granzyme A (GZMA) and RAS guanyl-releasing protein 1 (RASGRP1) were verified by CRISPR/Cas9-mediated knockdown. Knocking down expression of GZMA and RASGRP1 in attenuated macrophages led to a regain in their dissemination in Rag2/γC mice confirming their role as dissemination suppressors in vivo. We further evaluated the roles of GZMA and RASGRP1 in human B-lymphomas by comparing the transcriptome of 934 human cancer cell lines to that of Theileria-transformed bovine host cells. We confirmed dampened dissemination potential of human B-lymphomas that overexpress GZMA and RASGRP1. Our results provide evidence that GZMA and RASGRP1 have a novel tumour suppressor function in both T. annulata-infected bovine host leukocytes and in human B-lymphomas.
dc.publisherWiley
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/abs/10.1111/cmi.13255
dc.rightsArchived with thanks to Cellular Microbiology
dc.titleNovel tumour suppressor roles for GZMA and RASGRP1 in Theileria annulata-transformed macrophages and human B-lymphoma cells
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.contributor.departmentPathogen Genomics Laboratory
dc.identifier.journalCellular Microbiology
dc.rights.embargodate2021-08-24
dc.eprint.versionPost-print
dc.contributor.institutionLaboratoire de Biologie Comparative des Apicomplexes, Faculté de MédecineUniversité Paris Descartes - Sorbonne Paris Cité France
dc.contributor.institutionInserm U1016, Cnrs UMR8104Cochin Institute Paris France
dc.contributor.institutionCIPEM (Centre de Coalition, Innovation, et de prévention des Epidémies au Maroc)
dc.contributor.institutionKing Abdullah International Medical Research Center (KAIMRC), King Abdulaziz Medical City, Ministry of National Guard Health Affairs Jeddah Saudi Arabia
dc.contributor.institutionGlobal Station for Zoonosis ControlGlobal Institution for Collaborative Research and Education (GI-CoRE) , Hokkaido University, N20 W10 Kita-ku Sapporo Japan
kaust.personRchiad, ‍Zineb
kaust.personHaidar, Malak
kaust.personAnsari, Hifzur Rahman
kaust.personMfarrej, Sara
kaust.personBen Rached, Fathia
kaust.personKaushik, Abhinav
kaust.personPain, Arnab
dc.date.accepted2020-08-03
refterms.dateFOA2020-08-25T06:20:06Z
dc.date.published-online2020-09-29
dc.date.published-print2020-12


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