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dc.contributor.authorZhang, Peng
dc.contributor.authorMoretti, Manola
dc.contributor.authorAllione, Marco
dc.contributor.authorTian, Yuansi
dc.contributor.authorOrdonez-Loza, Javier
dc.contributor.authorAltamura, Davide
dc.contributor.authorGiannini, Cinzia
dc.contributor.authorTorre, Bruno
dc.contributor.authorDas, Gobind
dc.contributor.authorLi, Erqiang
dc.contributor.authorThoroddsen, Sigurdur T
dc.contributor.authorSarathy, Mani
dc.contributor.authorAutiero, Ida
dc.contributor.authorGiugni, Andrea
dc.contributor.authorGentile, Francesco
dc.contributor.authorMalara, N.
dc.contributor.authorMarini, Monica
dc.contributor.authorDi Fabrizio, Enzo M.
dc.date.accessioned2020-08-23T07:15:37Z
dc.date.available2020-08-23T07:15:37Z
dc.date.issued2020-08-20
dc.date.submitted2020-01-021
dc.identifier.citationZhang, P., Moretti, M., Allione, M., Tian, Y., Ordonez-Loza, J., Altamura, D., … Di Fabrizio, E. (2020). A droplet reactor on a super-hydrophobic surface allows control and characterization of amyloid fibril growth. Communications Biology, 3(1). doi:10.1038/s42003-020-01187-7
dc.identifier.issn2399-3642
dc.identifier.pmid32820203
dc.identifier.doi10.1038/s42003-020-01187-7
dc.identifier.urihttp://hdl.handle.net/10754/664752
dc.description.abstractMethods to produce protein amyloid fibrils, in vitro, and in situ structure characterization, are of primary importance in biology, medicine, and pharmacology. We first demonstrated the droplet on a super-hydrophobic substrate as the reactor to produce protein amyloid fibrils with real-time monitoring of the growth process by using combined light-sheet microscopy and thermal imaging. The molecular structures were characterized by Raman spectroscopy, X-ray diffraction and X-ray scattering. We demonstrated that the convective flow induced by the temperature gradient of the sample is the main driving force in the growth of well-ordered protein fibrils. Particular attention was devoted to PHF6 peptide and full-length Tau441 protein to form amyloid fibrils. By a combined experimental with the molecular dynamics simulations, the conformational polymorphism of these amyloid fibrils were characterized. The study provided a feasible procedure to optimize the amyloid fibrils formation and characterizations of other types of proteins in future studies.
dc.description.sponsorshipThe authors acknowledge financial support from King Abdullah University of Science and Technology for OCRF-2014-CRG and OCRF-2016-CRG grants and from Piedmont Region through European Funds for Regional Development (“Food Digital Monitoring” project). And Istituto di Cristallografia—Consiglio Nazionale delle Ricerche (IC-CNR) would like to thank the funding from MIUR (Italian Ministry for Education, University and Re-search) in the “PON Ricerca e Competitività 2007–2013” Program: ReCaS (Azione I—Interventi di rafforzamento strutturale, PONa3_00052, Avviso 254/Ric) and PRISMA (Asse II—Sostegno ll’innovazione, PON04a2_A).
dc.publisherSpringer Nature
dc.relation.urlhttp://www.nature.com/articles/s42003-020-01187-7
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleA droplet reactor on a super-hydrophobic surface allows control and characterization of amyloid fibril growth
dc.typeArticle
dc.contributor.departmentChemical Engineering Program
dc.contributor.departmentClean Combustion Research Center
dc.contributor.departmentCombustion and Pyrolysis Chemistry (CPC) Group
dc.contributor.departmentHigh-Speed Fluids Imaging Laboratory
dc.contributor.departmentMaterial Science and Engineering Program
dc.contributor.departmentMechanical Engineering Program
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.identifier.journalCommunications Biology
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionIstituto di Cristallografia – Consiglio Nazionale delle Ricerche (IC-CNR), Via Amendola 122/O, 70126 Bari, Italy.
dc.contributor.institutionDepartment of Physics, Khalifa University, P.O. Box: 127788Abu Dhabi, UAE.
dc.contributor.institutionDepartment of Modern Mechanics, University of Science and Technology of China, Hefei, Anhui 230026, China.
dc.contributor.institutionMolecular Horizon, Bettona, Italy.
dc.contributor.institutionNational Research Council, Institute of Biostructures and Bioimaging, Naples, Italy.
dc.contributor.institutionDepartment of electrical Engineering and Information Technology, University Federico II, Naples, Italy.
dc.contributor.institutionBIONEM lab, University Magna Graecia, Campus Salvatore Venuta, Viale Europa, 88100 Catanzaro, Italy.
dc.contributor.institutionMaterials and Microsystems Laboratory, Department of Applied Science and Technology, Politecnico di Torino, 10129 Torino, Italy.
dc.identifier.volume3
dc.identifier.issue1
kaust.personZhang, Peng
kaust.personMoretti, Manola
kaust.personAllione, Marco
kaust.personTian, Yuansi
kaust.personOrdonez-Loza, Javier
kaust.personTorre, Bruno
kaust.personThoroddsen, Sigurdur T.
kaust.personSarathy, Mani
kaust.personGiugni, Andrea
kaust.personDi Fabrizio, Enzo M.
kaust.grant.numberOCRF-2014-CRG
kaust.grant.numberOCRF-2016-CRG
dc.date.accepted2020-07-31
refterms.dateFOA2020-08-23T07:16:38Z
dc.date.published-online2020-08-20
dc.date.published-print2020-12


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This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
Except where otherwise noted, this item's license is described as This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.